人血清对幽门螺杆菌生长的影响

将幽门螺杆菌 (Hp)分别接种于含有新鲜羊血、不同状况人血清或小牛血清的培养基行微需氧培养 ,然后计数生长的菌落数。结果显示Hp可在含人血清或小牛血清培养基上生长 ,但生长效果略差于含羊血培养基 (P >0 .0 5 )。人血清抗Hp IgG抗体不影响Hp的生长 ,但未经补体灭活的人血清明显抑制Hp生长 (P <0 .0 1)。提示在条件受限的医院可用补体灭活的人血清或小牛血清替代羊血用于Hp培养 ;人血清对Hp的杀伤活性与抗Hp抗体无关 ,而与补体介导作用密切相关。

Effect of human serum on the growth of Helicobacter pylori

To investigate whether sheep blood could be replaced with human serum or fetal calf serum for Helicobacter pylori(Hp) culture, Hp was inoculated onto the culture plates containing 7% of sheep blood, human serum, or fetal calf serum respectively. The colonies were counted after 5 days of culture. Similar culture results were obtained in the plates containing sheep blood, human serum, or fetal calf serum respectively. No effect of anti Hp IgG antibody in human serum on the growth of Hp was found (P>0.05). However, heat inactivated human serum significantly suppressed the growth of Hp (P<0.01). Human serum or fetal calf serum may be used for Hp culture where sheep blood is not available. Bactericidal activity against Hp in human serum is mediated by complements.