糖尿病创面愈合与GM-CSF关系的研究

目的探讨糖尿病创面愈合与粒细胞巨噬细胞集落刺激因子（GM-CSF）的关系。方法70只C 57B L/6小鼠分为野生小鼠组（对照组,n=35）和糖尿病模型组（DM组,n=35）。腹腔麻醉后在背部中线两侧各制作0.8cm×0.8cm创面。创面动态摄像并于相应时间段取标本,观察创面组织愈合情况,同时计算创面愈合率;EL ISA法测定创面GM-CSF表达。结果创面形成后第3天起,DM组小鼠创面愈合率较对照组明显下降,以创面形成后7 d内变化最为明显;创面形成后第1天,两组小鼠创面GM-CSF表达均明显增高;创面形成后第1天和第3天,对照组小鼠创面GM-CSF表达显著高于DM组。结论GM-CSF的低表达可能与创面愈合早期炎症细胞浸润减少有关。

Relationship between wound healing of diabetes mellitus and granulocyte-macrophage colony-stimulating factor

Objective To study the relationship between wound healing of diabetes mellitus and granulocyte-macrophage colony-stimulating factor.Methods 70 C57BL /6 mice were divided into control group（n=35） and DM group（n=35）.The animals were made full thickness skin wound（0.8 cm ×0.8 cm） in each side of midline after deep anesthesia.The wound sites were digitally photographed to calculate the percentage of wound closure computer image analysis software.The content of GM-CSF in the wound was measured by ELISA.Results The wound healing in DM mice was significantly delayed compared with that in the control mice from the third day after wounding. In the DM mice, the levels of GM-CSF expression were markedly increased from the first day after wounding and declined to lower levels in the two groups. At the first day and third day after wounding, GM-CSF levels in DM mice were significantly lower than those in the control mice. Conclusion The delayed wound healing in DM mice may be lower expression of GM-CSF in wound site.