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| 枸杞多糖抑制人白血病细胞生长的研究 | |
| 引用本文： | 甘璐,王建华,张声华.枸杞多糖抑制人白血病细胞生长的研究[J].卫生研究,2001,30(6):333-335. |
| 作者姓名： | 甘璐 王建华 张声华 |
| 作者单位： | 1. 华中科技大学生命科技学院, 2. 山东农业大学食品工程学院 3. 华中农业大学食品科技系 |
| 摘 要： | 用体外培养法研究枸杞多糖 (LBP X)对人白血病HL 6 0细胞生长的抑制作用并探讨其作用机制。结果表明 :LBP X(2 0、10 0、5 0 0和 10 0 0mg L)与HL 6 0细胞共培养 48h后 ,LBP X抑制HL 6 0细胞增殖呈剂量依赖性关系 ,且可降低HL 6 0细胞的膜流动性 ,HL 6 0细胞的DNA琼脂糖凝胶电泳可见明显的“DNAladder” ,TUNEL方法检测呈阳性。这提示诱导HL 6 0细胞凋亡是LBP X抗肿瘤的重要机制 |
| 关 键 词： | 枸杞多糖 人白血病细胞系 抗肿瘤 细胞凋亡 |
| 文章编号： | 1000-8020(2001)06-0333-03 |
| 修稿时间： | 2000年12月28 |
Inhibition the growth of human leukemia cells by Lycium barbarum polysaccharide |
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| Gan Lu,Wang Jianhua,Zhang Shenghua School of Life Science and Technology,Huazhong University of Science and Technology,Wuhan ,China.Inhibition the growth of human leukemia cells by Lycium barbarum polysaccharide[J].Journal of Hygiene Research,2001,30(6):333-335. | |
| Authors: | Gan Lu Wang Jianhua Zhang Shenghua School of Life Science and Technology Huazhong University of Science and Technology Wuhan China |
| Institution: | School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China. |
| Abstract: | The effect and the mechanism of Lycium barbarum polysaccharide (LBP-X) on inhibiting the growth of human leukemia HL-60 cells were examined. LBP-X(20, 100, 500, 1000 mg/L) could inhibit the growth of HL-60 cells in dose-dependent manner and decrease the membrane fluidity of the cell. Agarose gel electrophoresis of DNA from the cells treated with LBP-X revealed a "DNA ladder" and positive TUNEL test. The results showed that the apoptosis of HL-60 cells induced by LBP-X maybe its important mechanism on anti-tumorgenesis. |
| Keywords: | Lycium barbarum polysaccharide human leukemia cell line antitumor apoptosis |
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