Synthetic genes: a tool for identifying human papillomavirus genotypes by hybridization and polymerase chain reaction-based assays |
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| Authors: | Orjuela Karen Amador Catalina Patarroyo Manuel A |
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| Affiliation: | Molecular Biology Department, Fundacion Instituto de Inmunologia de Colombia, Bogota, Colombia. |
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| Abstract: | Obtaining positive polymerase chain reaction (PCR) controls for human papillomavirus (HPV) diagnostic tests has been difficult because of prevalence variation in different geographic regions of each high-risk viral type. Overlapping oligonucleotides were designed for HPV-18, HPV-31, HPV-45, and HPV-58 type-specific (TS) sequences. Synthetic HPV viral genes were constructed by 2-step assembly PCR for accurately diagnosing TS HPV infection. |
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| Keywords: | HPV, Human papillomavirus HR-HPV, High-risk human papillomavirus PCR, Polymerase chain reaction TS, Type specific |
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