利用BIOFOSUN系统鉴定常见曲霉

目的评估BIOFOSUN系统鉴定临床常见曲霉的可靠性。方法采用常规形态学方法及分子生物学方法测定菌株rDNA保守区基因序列鉴定77株临床收集的曲霉,并与BIOFOSUN系统鉴定结果比较。结果BIOFOSUN系统对构巢曲霉、杂色曲霉、寄生曲霉、棒曲霉、泡盛曲霉、焦曲霉、黄柄曲霉及日本曲霉种的鉴定符合率均为100%,对黄曲霉、烟曲霉、黑曲霉、土曲霉的鉴定符合率分别为92%、85%、75%及70%,对于土曲霉的鉴定符合率相对较差,利用BIOFOSUN系统提供的形态学图谱,观察其具有特征性的菌落外型及色素,可以帮助鉴定。使用BIOFOSUN系统可以将一些形态学特征接近,且又不能通过rDNA基因测序方法进行区分的曲霉作出鉴定。结论BIOFOSUN系统可以利用计算机进行数据分析,自动化和标准化程度高,大大简化鉴定程序,为曲霉的标准化鉴定提供了一个有效的方法。

Identification of common Aspergillus using BIOFOSUN system

Objective To evaluate the reliability of BIOFOSUN system in identifying common Aspergillus.Methods 77 clinical isolates of Aspergillus were identified by routine morphological and molecular biological sequencing of rDNA conservative areas.The results of identification were compared with those from BIOFOSUN system.Results The coincidence rates of BIOFOSUN system identifying Aspergillus nidulans,Aspergillus fumigatus,Aspergillus parasiticus,Aspergillus clavatus,Aspergillus awamori,Aspergillus ustus,Aspergillus flavipes and Aspergillus japonicus were all 100%.The coincidence rates of identifying Aspergillus flavus,Aspergillus fumigatus,Aspergillus niger and Aspergillus terreus were 92%,85%,75% and 70% respectively.The coincidence rate of identifying Aspergillus terreus was relatively low,but the shape and pigment of the colony from BIOFOSUN system morphological images were characteristic,which could help identification.BIOFOSUN system could differentiate the strains with similar morphological feature,which could not be differentiated by rDNA sequencing.Conclusions BIOFOSUN system can analyze data through computer,which is highly automatic and standard.This system can simplify the procedure of identification consumedly and provide an effective method for standardized identification of Aspergillus.