c—met—SF／HGF基因表达对肝癌细胞转移能力的影响

目的：研究c-met-SF/HGF基因表达对肝癌细胞转移能力的影响。方法：采用RT－PCR及Wiestern Blot法检测肝癌细胞株MHCC－1、HepG2,Hep3B,SMMC7721的met-SF/HGF的表达，继而用c-met多克隆抗体阻断met-SF/HGF的信号传导，观察细胞各项能力的改变。结果：转移能力高的细胞株（MHCC－1）c-met的表达量远高于转移能力低的细胞株，并伴有配体SF／HGF的自分泌；MHCC－1上清液能刺激SMMC7721细胞增殖并使其运动能力增强；该作用被抗c-met抗体特异性阻断，当被阻断后，细胞生长及运动能力下降。结论met-SF/HGF 基因表达在肝癌转移中起重要的作用，阻断c-met-SF/HGF的信号传导将降低肿瘤转移的能力。

Influence on Metastatic Capability of Hepatocellular Carcinoma Cells by c-Met-SF/HGF Expression

Objective To clarify the function of c met and its ligand HGF expression in hepatocellular carcinoma.Methods RT PCR and Western Blot were used to examine the expression of c met and SF/HGF in hepatocellular carcinoma cell line MHCC 1,HepG2,Hep3B and SMMC7721.SMMC7721 was also stimulated by the conditioned medium of MHCC 1,and the stimulation was inhibited by c met polyclonal antibody to observe cell mobility and proliferation.Results Cell mobility of hepatocellular carcinoma was relative to its met SF/HGF expression.The expression in cell line with higher potential of metastasis(MHCC 1) was much stronger than in those with lower potential of metastasis and was with SF/HGF autocrine.An increase of proliferation and mobility happened in SMMC7721 after it was stimulated by conditioned medium of MHCC 1.Such functions could be specifically blocked by anti c met polyclonal antibody.Conclusion c met SF/HGF autocrine and paracrine play an important role in the metastasis potential of hepatocellular carcinoma.Inhibition of c met SF/HGF signal transduction system may reduce the metastasis of hepatocellular carcinoma.