|
|||||
|
|
| Rho激酶通路的激活对血管收紧缩张的素作Ⅱ用诱导的人气道平滑肌细胞 | |
| 引用本文： | Li N,Meng Y,Cheng YX,Niu Y,Shen B,Li X,Lai WY. Rho激酶通路的激活对血管收紧缩张的素作Ⅱ用诱导的人气道平滑肌细胞[J]. 南方医科大学学报, 2011, 31(8): 1416-1420 |
| 作者姓名： | Li N Meng Y Cheng YX Niu Y Shen B Li X Lai WY |
| 作者单位： | 1. 南方医科大学南方医院 呼吸科 2. 南方医科大学南方医院 急诊科,广东广州,510515 3. 南方医科大学南方医院 心内科,广东广州,510515 |
| 基金项目： | 南方医院院长基金(2009B007) |
| 摘 要： | 目的探讨Rho激酶通路的激活对血管紧张素I(IAng II)诱导的人气道平滑肌细胞(human airway smooth musclecells,HASMCs)收缩的作用及其机制。方法原代培养人气道平滑肌细胞,分为对照组、AngII组、AngII+伊贝沙坦[AngII1型受体(AT1R)阻断剂,IRB]组、AngII+Y-27632(Rho激酶抑制剂)组分别用胶原收缩法、免疫荧光等从细胞形态检测AngII对细胞收缩的影响及Western Blot在蛋白水平上检测Rho激酶通路蛋白的变化。结果 Y-27632、伊贝沙坦均可显著抑制AngI(I10-7 mol/L)诱导的人气道平滑肌细胞收缩,两组的抑制作用无显著差异(P>0.05);Y-27632对AngII诱导的Rho激酶底物磷酸化moesin的表达的抑制作用显著高于伊贝沙坦(P<0.05)。结论 AngII诱导的HASMCs收缩可能与Rho激酶通路的激活有关。 |
| 关 键 词： | 人气道平滑肌细胞 收缩 血管紧张素II AT-1受体抑制剂 Rho激酶通路 |
Activation of Rho-kinase pathway is involved in angiotensin II-induced contraction of human airway smooth muscle cells |
|
| Li Ning,Meng Ying,Cheng Yuan-xiong,Niu Yi,Shen Bin,Li Xu,Lai Wen-yan. Activation of Rho-kinase pathway is involved in angiotensin II-induced contraction of human airway smooth muscle cells[J]. Journal of Southern Medical University, 2011, 31(8): 1416-1420 | |
| Authors: | Li Ning Meng Ying Cheng Yuan-xiong Niu Yi Shen Bin Li Xu Lai Wen-yan |
| Affiliation: | LI Ning1,MENG Ying1,CHENG Yuan-xiong1,NIU Yi1,SHEN Bin1,LI Xu2,LAI Wen-yan31Department of Respiratory Diseases,2Departmentof Emergency Medicine,3Department of Cardiology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,chin |
| Abstract: | Objective To investigate of the regulatory effect of Rho-kinase pathway activation on angiotensin II(Ang II)-induced contraction of human airway smooth muscle cells(HASMCs) in vitro.Methods Cultured primary HASMCs were divided into control group,AngII group,AngII + irbesartan group and AngII + Y-27632 group with corresponding treatment.AngII-induced contraction of HASMCs was evaluated using collagen gel lattices and observed morphologically using immunofluorescence assay.Western Blotting was significantly p... |
| Keywords: | human airway smooth cells contraction angiotensin II inhibitor of Ang II type 1 recepetor Rho-kinase pathway |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |