| H~+-ATP酶定位的电镜细胞化学观察 |
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| 引用本文： | Luo SQ,Ke ZY,Lu YM. H~+-ATP酶定位的电镜细胞化学观察[J]. 南方医科大学学报, 2011, 31(8): 1431-1433 |
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| 作者姓名： | Luo SQ Ke ZY Lu YM |
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| 作者单位： | 1. 南方医科大学细胞生物学教研室,广东省骨与软骨再生医学重点实验室
2. 电镜室,广东广州,510515 |
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| 基金项目： | 广东省自然科学基金(101510515D1000040) |
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| 摘 要： | 目的用电子显微镜细胞化学方法观察H+-ATP酶在细胞器中的位置。H+-ATP酶不仅定位在溶酶体膜,而且定位在Wistar大鼠近曲小管上皮细胞的细胞膜、核膜上。上述结果表明用电镜细胞化学方法以形态学表现形式证实H+-ATP酶定位在细胞内膜相结构的学说。
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| 关 键 词： | H+-ATP酶 电子显微镜细胞化学 |
Ultracytochemical observation of the intracellular localization of H+-adenosine triphosphatase |
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| Luo Shen-qiu,Ke Zhi-yong,Lu Yan-meng. Ultracytochemical observation of the intracellular localization of H+-adenosine triphosphatase[J]. Journal of Southern Medical University, 2011, 31(8): 1431-1433 |
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| Authors: | Luo Shen-qiu Ke Zhi-yong Lu Yan-meng |
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| Affiliation: | LUO Shen-qiu1,KE Zhi-yong1,LU Yan-meng2 1Department of Cell Biology,2Laboratory of Electron Microscopy,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | Objective To observe the ultracytochemical localization of H+-adenosine triphosphatase(H+-ATPase) in the cell organelles.Methods The localization of H+-ATPase in the cell organelles was observed in the hepatocytes and renal cells of Wistar rats using routine ultracytochemical methods.Results H+-ATPase activities were observed on the lysosomal membrane and nuclear envelope of the hepatocytes and proximal tubule epithelial cells of the nephron in Wistar rats.Conclusion This finding supports the hypothesis tha... |
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| Keywords: | H -adenosine triphosphatase ultracytochemical localization |
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