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| 结直肠癌转移相关基因PCR芯片的建立及初步应用 | |
| 引用本文： | 肖小琴,唐娜,刘超,王红霞,蒋强,邓永键,丁彦青. 结直肠癌转移相关基因PCR芯片的建立及初步应用[J]. 南方医科大学学报, 2011, 31(7): 1169-1174 |
| 作者姓名： | 肖小琴 唐娜 刘超 王红霞 蒋强 邓永键 丁彦青 |
| 作者单位： | 南方医科大学南方医院病理科;南方医科大学基础医学院病理学系;深圳市观澜人民医院病理科; |
| 基金项目： | 国家自然科学基金(81071735); 广东省科技计划项目(2010B031500012); 广东省高等学校科技创新重点项目(cxzd1016)~~ |
| 摘 要： | 摘要：目的建立同时检测表达多个结直肠癌转移相关基因的PCR芯片，用于诊断结直肠癌转移和预测转移。方法通过 严格设计基因引物，探索各基因同时达到特异性扩增的PCR反应条件；应用基因芯片筛选获得的29个结直肠癌转移相关 基因，结合3个看家基因，建立同时检测32个基因的PCR芯片；应用该芯片检测不同转移潜能的结直肠癌细胞和临床直肠 癌组织、癌旁正常粘膜。结果成功构建同时检测32个基因的PCR芯片，各基因均获得特异性扩增；29个转移相关基因 PCR芯片检测结果与基因芯片检测结果的吻合率为86%；PCR芯片检测伴有转移的结直肠原发癌与不伴有转移的结直肠 癌组织，结果显示14个基因表达一致，15个基因表达有差异。结论结肠癌转移PCR芯片结果可靠，可用于预测结直肠癌 转移的研究。 |
| 关 键 词： | PCR芯片 结直肠癌 肿瘤转移相关基因 分子预测 |
Construction of a PCR chip for detecting genes associated with metastatic colorectal cancer and its preliminary application |
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| XIAO Xiao-qin,TANG Na,LIU Chao,WANG Hong-xia,JIANG Qiang,DENG Yong-jian,DING Yan-qing. Construction of a PCR chip for detecting genes associated with metastatic colorectal cancer and its preliminary application[J]. Journal of Southern Medical University, 2011, 31(7): 1169-1174 | |
| Authors: | XIAO Xiao-qin TANG Na LIU Chao WANG Hong-xia JIANG Qiang DENG Yong-jian DING Yan-qing |
| Affiliation: | XIAO Xiao-qin 1,2,TANG Na 1,LIU Chao 1,WANG Hong-xia 1,JIANG Qiang 1,DENG Yong-jian 1,DING Yan-qing 1 1 Department of Pathology,Nanfang Hospital/School of Basic Medical Science,Southern Medical University,Guangzhou 510515,China,2 Department of Pathology,Shenzhen Guanlan People's Hospital,Shenzhen 518110 |
| Abstract: | Objective To construct a PCR chip with a gene panel for predicting and diagnosing metastatic colorectal cancer.Methods The PCR chip was constructed by integrating 29 genes related to colorectal cancer metastasis identified by gene chip analysis and 3 housekeeping genes into a gene panel.The PCR chip was used for detecting the mRNA expressions of the integrated genes in colorectal cell lines,cancerous specimen and adjacent normal mucosa.The primers for amplification were refined and optimized by several roun... |
| Keywords: | PCR chip colorectal cancer genes associated to metastasis molecular prognosis |
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