槲皮素对成年大鼠局灶性脑缺血后侧脑室室管膜下区神经干细胞增殖的影响

摘要：目的观察槲皮素对成年大鼠局灶性脑缺血后侧脑室室管膜下区（SVZ）细胞增殖的影响。方法线栓法制作大鼠右
侧大脑中动脉阻塞模型，术后6 h腹腔注射槲皮素（50 mg/kg，1次/3 d），术后4 h腹腔注射BrdU（50 mg/kg，1次/d），分别于
缺血第7、14、21天采用免疫荧光染色观察侧脑室SVZ BrdU阳性细胞数。结果脑缺血第7天，缺血侧SVZ BrdU阳性细
胞较假手术组明显增多，第14天达峰值，第21天减少（P<0.01）。槲皮素组第7天时，缺血侧SVZ BrdU阳性细胞亦明显增
多，并随着缺血时间延长明显增加；与缺血组比较，槲皮素组7、14 和21 d 缺血侧SVZ BrdU阳性细胞数均显著增加（P<
0.01），至21 d仍保持高水平。结论槲皮素可维持成年大鼠局灶性脑缺血后侧脑室SVZ的细胞增殖在较高水平。

Effect of quercetin on neural stem cell proliferation in the subventricular zone of rats after focal cerebral ischemia-reperfusion injury

Objective To study the effect of quercetin on the proliferation of neural stem cells in the subventricular zone(SVZ) of rats after focal cerebral ischemia.Methods An adult rat model of middle cerebral artery occlusion(MCAO) model was established by placement of an intraluminal filament at the origin of the MCA.Quercetin was administered intraperitoneally in the rats at a dose of 50 mg/kg every 3 days starting at 6 h after MCAO,and BrdU(50 mg/kg daily) was also injected intraperitoneally starting at 4 h afte...