猴头菌丝体对大鼠慢性胃损伤的保护作用及机制研究

目的　探讨猴头菌丝体对慢性胃损伤大鼠的保护作用及机制，为其用于慢性胃损伤的预防和治疗提供依据。方法　40只大鼠随机分为空白对照组、模型对照组、阳性对照组（猴头菌片，187.5mg/kg·bw）、猴头菌丝体低、高剂量组（834mg/kg·bw，3333mg/kg·bw），每组8只。空白对照组每日上午经口灌胃去离子水，其余各组经口灌胃0.3 %的碘乙酰胺；空白对照组和模型对照组每日下午经口灌胃去离子水，其余各组经口灌胃相应浓度的菌丝体浸出液或猴头菌片水溶液。干预30d麻醉后放血处死大鼠，称体重及重要脏器重量。观察胃黏膜大体变化并进行组织病理学检查，检测血清中超氧化物歧化酶（SOD）、髓过氧化物酶（MPO）、一氧化氮（NO）、一氧化氮合酶（NOS）、肿瘤坏死因子-α（TNF-α）的活性/含量。结果　各组动物一般状况良好，模型对照组、阳性对照组及两个受试物组体重增重和重要脏器的脏器系数与空白对照组比较均无统计学差异（P＞0.05）。与空白对照组相比，模型对照组胃黏膜病理损伤较严重，受试物两剂量组的损伤程度有所减轻。与模型对照组相比，猴头菌丝体高剂量组大鼠的SOD活力升高，且受试物两剂量组的MPO、TNF-α、NOS、NO含量或活力均下降。结论　猴头菌丝体可缓解由碘乙酰胺导致的大鼠慢性胃损伤，其机制可能与提高机体抗氧化应激能力以及抑制炎症反应有关。

Protective effects and mechanism of hericium mycelium on chronic gastric injury in rats

Objective　To investigate the protective effects and mechanism of hericium mycelium on chronic gastric injury in rats, and to provide basis for preventing and treating of chronic gastric injury. [WTHZ]Methods　40 rats were randomly divided into blank control group, model control group, positive control group (hericium mycelium tablet, 187.5 mg/kg.bw), low dose group of hericium mycelium and high dose group of hericium mycelium (834 mg/kg.bw, 3333 mg/kg.bw), with 8 rats in each group. In the mornings, the blank control group was orally gavaged with deionized water, the remaining groups were orally administered with 0.3% iodoacetamide. In the afternoons, the blank control group and model control group were orally administered with deionized water, the remaining groups were orally administered with the corresponding concentration of mycelium leachate or hericium erinaceus tablets water solution. After 30d intervention, the rats were sacrificed by exsanguination after anesthesia, and then body weight and the weight of vital organs were determined. The general changes and the histopathology of gastric mucosa were observed and serum superoxide dismutase (SOD), myeloperoxidase (MPO), nitric oxide(NO), nitric oxide synthase (NOS), tumor necrosis factor (TNF-α) activity or content were tested. [WTHZ]Results　The animals in each group were generally in good condition. There was no significant difference in body weight gain or organ coefficient between the model control group, positive control group and the two test substance groups compared with the blank control group (P>0.05). Compared with the blank control group, the pathological changes of gastric mucosa were more serious in the model group, and the lesion degree in the two dose groups of the test substance was lessened. Compared with the model control group, the SOD activity of rats in the high dose group of hericium mycelium increased, and the content or activity of MPO, TNF-α, NOS and NO in the two dose groups decreased. [WTHZ]Conclusion　Hericium mycelium can alleviate chronic gastric injury induced by iodoacetamide in rats. The mechanism may be related to improving the antioxidative capability and inhibiting inflammatory reaction.