低电压电化学疗法对头颈部肿瘤裸鼠移植瘤抑制作用的研究

目的：探讨低电压电化学疗法对头颈部肿瘤的治疗意义。方法：于裸鼠下颌皮下接种人头颈部鳞状上皮细胞癌YCU—N861细胞系细胞，制成移植瘤模型。局部注射博来霉素（BLM）20μg／只，继以50V、100V的电压刺激（EP），观察肿瘤生长情况，并测量其体积。9d后取肿瘤组织，常规苏木精-伊红染色及免疫组织化学染色后观察。结果：BLM和EP联合应用能明显抑制肿瘤细胞的增殖，联合治疗组（BLM＋EP）与BLM组、EP组和空白对照组比较，均差异有统计学意义（均P〈0．01）；BLM＋EP组肿瘤生长发育迟缓，明显受到抑制，组织学表现为坏死出血征象，有丝分裂指数和免疫组织化学Ki67染色标记指数分别为9．90±3．48、2．28±2．61，与BLM组（34．33±6．60、6．41±3．59）、EP组（58．75±8．87、8．85±3．25）及对照组（73．25±11．95、11．10±3．67）比较，差异有统计学意义（均P〈0．01）。结论：低电压电化学疗法在头颈部肿瘤的治疗方面具有广阔的应用前景。

Studies on inhibitive effect of low-voltage electrochemotherapy on head and neck cancer-bearing nude mice

Objective:To analyse the therapic effect of low-voltage electrochemotherapy (EP) on the head and neck cancer.Method:YCU-N861 human head and neck cancer cells were subcutaneously inoculated in the neck of nude mice to establish tumor-bearing models. Then the mice were treated with bleomycin(BLM, 20 μg per mouse), accompanied with electric stimulation(50 V or 100 V).Result:BLM treatment applied with EP can significantly inhibite the proliferation of tumor cells than BLM or EP treatment seperately(P<0.01) . The tumor volume, the mitotic index (MI) and the labeling index (LI) of Ki67 in BLM+EP group were all obviously decreased than BLM group, EP group and control group (P<0.01).Conclusion:Low-voltage electrochemotherapy can offer promising mothod in the local treatment of head and neck cancer.