亚低温对外源性骨髓基质细胞迁移、增殖的影响

目的:了解亚低温对外源性骨髓基质细胞迁移、增殖的影响,探讨亚低温可能的脑保护机制。方法:建立大鼠局灶性脑梗塞模型,从侧脑室注射骨髓基质细胞,给予亚低温干预,观察移植后第14、28天低温组和常温组神经功能评分、梗死灶周围BrdU(+)细胞数。结果:移植后第14和28天,亚低温组大鼠神经缺损评分低于同时间点的对照组评分,移植第14天多数标记的骨髓基质细胞细胞已经迁移至梗死灶周围,第14和28天亚低温组较常温组梗死灶周围有更多的BrdU(+)。结论:亚低温可以促进骨髓基质细胞的迁移和(或)增殖,对局灶性脑缺血有神经保护作用。

Effect of Mild Hypothermal on the Migaration and Proliferation of Bone Marrow Stromal Cells in Rats

Objective:To study the effect of mild hypothermal on the migaration and proliferation of BMSCs in rats.Methods:The models of middle cerebral artery occulusion(MCAO) were established in rats.After being purified proliferated and labbeld with BrdU,the BMSCs were injected into the lateral ventricles of the models.All rats were randomly assigned to the mild hypothermal group and control group.On 14 d and 28 d after injected,the recovery the neurological function in rats assayed through neurological scoring and immunohistochemical method was applied to observe the migration and generation of transplanted BMSCs in brain.Results:The rats treated by mild hypothermal had significantly lower NSS scores compared with those of control group(P<0.05).On 14 d,BrdU positive cells were migrated to ischemal infarction area.There are more BrdU positive cells assembled in ischemic infarction area in mild hypothermal group.Conclusion:Mild hypothermal can promote the migaration and(or) generation of bone marrow stromal cells in rats.