| 散发性病毒性脑炎免疫组化和原位杂交研究 |
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| 引用本文： | 向传喜,夏学锋,许念桂,彭隆祥,施启迈.散发性病毒性脑炎免疫组化和原位杂交研究[J].中南大学学报(医学版),1995(6). |
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| 作者姓名： | 向传喜 夏学锋 许念桂 彭隆祥 施启迈 |
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| 作者单位： | Xiang Chuanxi; Xia Xuefeng; Xu Niangui; Peng Longxiang(Laboratory of
Electron Microscopy，Hunan Medical University)Shi Qimai(Department of Pathology，Hunan
Medical University) |
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| 摘 要： | ２０例临床诊断为散发性病毒性脑炎的尸检和活检脑组织，通过单纯疱疹病毒多克隆抗体免疫组化和生物素标记单纯疱疹病毒ＤＮＡ探针原位杂交研究，５例诊断为单纯疱疹病毒脑炎。结果说明：单纯疱疹病毒是国内散发性病毒性脑炎的重要病原；免疫组化和原位杂交是诊断单纯疱疹脑炎的两种敏感方法。
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| 关 键 词： | 人疱疹病毒 脑炎病毒 免疫组织化学 组织细胞化学 原位杂交 |
STUDY ON SPORADIC VIRAL ENCEPHALITIS BY IMMUNOHISTOCHEMISTRY AND IN SITU
HYBRIDIZATION |
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| Xiang Chuanxi, Xia Xuefeng, Xu Niangui, Peng Longxiang.STUDY ON SPORADIC VIRAL ENCEPHALITIS BY IMMUNOHISTOCHEMISTRY AND IN SITU
HYBRIDIZATION[J].Journal of Central South University (Medical Sciences)Journal of Central South University (Medical Sciences),1995(6). |
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| Authors: | Xiang Chuanxi Xia Xuefeng Xu Niangui Peng Longxiang |
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| Abstract: | utopsy and biopsy specimens from twenty patients with clinically
diagnosed sporadicviral encephalitis were studied by histopathologic observation,and
investigated for herpessimplex virus(HSV)by immunohistochemistry with a polyclonal
antiserum and by in situDNA hybridization(ISH)with a biotinylated DNA probe. Five cases were
diagnosed asHSV encephalitis.The results demonstrated that HSV was the important etiologic
agent ofsporadic viral encephalitis in China,both immunohistochemistry and ISH are sensitive
tech- niques to demonstrate HSV in stiu. |
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| Keywords: | herpesvirus hominis encephalitis viruses immunohistochemistry histocytochemistry in situ hybridization |
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