The effect of processing and cryopreservation on nucleated umbilical cord blood cells |
| |
Authors: | Bayer-Zwirello Lucy A Hoffman Despina E Adams Lorrie A Wilder Paul T Reece Margaret T |
| |
Affiliation: | Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, St. Elizabeth's Medical Center, Boston, MA 02135, U.S.A. zwirello@massmed.org |
| |
Abstract: | Recovery of nucleated cord blood cells after storage in liquid nitrogen was evaluated. Red cells were depleted using Ficoll-Paque or Puregene red cell lyses. Freeze Medium contained 10% dimethylsulfoxide and 20% serum for cryoprotection. Recovery of the original cell population remaining serviceable for fluorescence activated cell sorting (FACS) was 12 +/- 10% (average +/- standard deviation), with a range of 1% to 55%. Viability measured by FACS analysis after freezing was significantly lower than that of the same specimens prior to freezing, 62 +/- 20% compared to 91+/-11% (p<0.001). Percentage CD45+34+ cells were the same for fresh and frozen cells. Gestational age at which specimens were collected had no effect on the percent cells carrying the CD45+34+ markers. We conclude that better cryoprotective supplements are needed to insure consistent high recovery of viable nucleated umbilical cord blood cells after preservation in liquid nitrogen. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|