血清淀粉酶同工酶谱诊断胰胆管连接异常的初步临床应用

目的 通过血清淀粉酶同工酶谱检测诊断伴有胆管轻度扩张的胰胆管连接异常。方法 采仪式 8例胆管轻度扩张并经术中造影证实PBM的患儿术前、术后血清，另取8例正常儿童血清作对照，应用不连续缓冲系统苤膜电泳法行酶谱分析。结果 政党组仅存在P1和P2，PBM患儿术前血清淀粉酶同工酶波峰除有P1和P2外，还出现P3，P4、P5等异常波峰，无优势波峰，该血清酶学异常在胰胆分流术后基本消失，结论 PBM患儿即使仅伴有胆管轻度扩张。其血清淀粉酶同工酶谱仍存在异常，具一定特异性，有助于PBM的临床诊断。

CLINICAL USAGE OF SERUM AMYLASE ISOENZYMES TO DIAGNOSE PANCREATICOBILIARY MALJUNCTION WITH MILD BILIARY DILATATION

Objective To evaluate the diagnostic value of serum amylase isoenzymes for the pancreaticobiliary maljunction (PBM) with mild biliary dilatation (BD).Methods The preoperative,postoperative serum from 8 children suffering PBM with mild BD and serun from 8 normal children as controls were studied.Electrophosresis in discontinuous buffer system was applied for the presenting patterns and fractions of the pancreatic amylase isoenzymes.Results The pattern of pancreatic isoenzymes in preoperative serum manifested P1-P5 without predominant fraction,while in normal controls,there were only P1 and P2.After operation,these abnormal changes in PBM disappeared.Conclusion There are remarkable changes in serum amlyase isoenzymes from PBM.These changes may be of diagnostic significance of PBM.