TLR2和TLR4在原发性肝癌中的表达

目的：本研究通过检测原发性肝癌Toll样受体2（TLR2）和Toll样受体4（TLR4）的表达，分析其与临床病理生理因素的关系，探讨TLR2，TLR4在原发性肝癌疾病过程中的可能作用。方法：采用免疫组化法和实时荧光定量PCR分别在蛋白水平及mRNA水平检测原发性肝癌组织和配对癌旁组织TLR2、TLR4的表达。结果：原发性肝癌组织在蛋白水平和mRNA水平TLR2和TLR4的表达均明显低于癌旁组织（P<0.01）。但免疫组化结果显示不论是肝癌组织还是癌旁组织，TLR2和TLR4的表达均明显高于正常肝组织（P<0.01，P<0.05），有门静脉分支癌栓的患者癌组织TLR4的表达强度低于无门静脉分支癌栓的患者（P<0.05）。结论：肝癌组织TLR2和TLR4的表达与癌旁组织相比受到相对抑制，但高于正常肝组织，TLR2和TLR4信号途径可能参与了原发性肝癌的病理过程。

Expression of TLR2 and TLR4 in hepatocellular carcinoma

AIM: To investigate the expression of TLR2 and TLR4 in hepatocellular carcinoma (HCC), and to analyze their correlations to clinicopathologic features of HCC. METHODS: The protein and mRNA levels of TLR2 and TLR4 in HCC and para-tumor tissue were determined by immunohistochemistry and real-time fluorescence quantitative PCR (RFQ-PCR). RESULTS: The protein and mRNA levels of TLR2 and TLR4 in HCC were lower than those in para-tumor tissue (P<0.01). At protein level, the intensities of TLR2 and TLR4 expression in HCC and para-tumor tissue were significantly higher than those in normal liver tissue (P<0.01 and P<0.05). Furthermore, the expressions of TLR2 and TLR4 in HCC with cancer embolism in portal vein were weaker than those in HCC without cancer embolism (2= 9.458,P<0.05). CONCLUSION: The intensities of TLR2 and TLR4 expression are lower in HCC than those in para-tumor tissue, whereas higher than those in normal liver tissue. TLR2 and TLR4 signals might take part in the course of HCC.