复方氨酚烷胺片中胆红素的HPLC测定方法研究

目的探讨复方氨酚烷胺片中胆红素的测定方法。方法用高效液相色谱法对胆红素进行含量测定，以DiamonsilTM c18（4．6mm×250mm，5μm）为分析柱，四氢呋喃-乙腈-0．1％醋酸铵（pH4．7）（42：22：36）为流动相；流速：1．0ml·min^-1；检测波长：452nm；进样量20μl。结果胆红素对照品在0．6555～13．11μg·ml。范围内，浓度与峰面积呈良好的线性关系，r=0．9995，平均回收率为98．8％，RSD=0．5％（n=9）。结论本法简便，准确，专属性强，可以作为该制剂的质量控制方法。

Determination Content of bilirubin in Compound Paracetamol and Amantadine Hydrochloride Tablets by HPLC

Objective To establish a method for the determination of bilirubin in Compound Paracetamol and Amantadine Hydrochloride Tablets.Methods To establish a method for the determination of bilirubin. The HPLC was conducted on a DiamonsiITM C18 ( 4.6mm ~ 250 mm,5μ m ) with tetrahydrofuran-acetonitrile-0.1% ammonium acetate ( pH4.7 ) ( 42:22: 36 ) as mobile phase, detected at 452nm;flow rate l.0ml-min-1.Result it was used the external standard method. The calibration curve of bilirubin was linear with 0.655 5~13.11 μ g · ml-1 ,(r=0.999 5),The average recovery of bilirubin was 98.8% with RSD=0.5% ( n=9 ).Concluslon The method is simple,accurate, strong specificity and suitable for the determination of bilirubin in Compound Paracetamol and Amantadine Hydrochioridc Tablets.