用随机扩增多态DNA(RAPD)技术研究不同地理株埃及伊蚊的分化

目的 用RAPD技术对实验室饲养的广东、海南、台湾和印尼Baro等4个不同地理株的8只雌蚊进行随机扩增多态DNA分析。方法 随机扩增多态性DNA(RAPD)技术。结果 选用20个随机引物进行扩增，有8个引物表现清晰的RAPD谱带并呈显著多态性。UPGMA法构建的分子系统树表明埃及伊蚊4个地理株之间存在着一定程度的遗传分化。结论 用RAPD方法可以区分不同地理株埃及伊蚊。

Differentiation of Aedes aegypti with Genetic Polymorphisms Detected By the Random Amplified Polymorphism DNA Polymerase Chain Reaction (RAPD-PCR)

Objective This paper intends to study the genetic variation of Aedes aegypti from Hainan,Guangdong and Taiwan of China and Baro of Indonesia respectively.Methods Random-amplified polymorphic DNA polymerase chain reaction(RAPD-PCR) was used to analyze the DNA polymorphism and unweighted pair group method with arithmetic mean (UPGMA) was used as cluster analysis for DNA of Aedes aegypti.Results (1)Out of total 113 RAPD bands received,109( 96.46) presented the polymorphism.(2)The cluster of analysis by UPGMA showed elected samples can be divided into different groups:Hainan,Guangdong,Taiwan strain of China and Baro strain of Indonesia.The inheritic distance of Guangdong strain and Baro strain was the closest.The Taiwan strain was relatively independent group.Conclusion RAPD is an useful method to detected and classify Aedes aegypti.The analysis of polymorphic DNA reveals that there have inheritic differentiation in different population of Aedes aegypti.