恒河猴感染华支睾吸虫的初步观察

用恒河猴感染华支睾吸虫获得成功。两只恒河猴获虫率分别为9.9％及31.59％。所得虫体都已发育成熟。感染后30天所得虫体平均长5.15mm,宽1.005mm。感染后48天所得虫体平均长8.0625mm,宽1.34mm。两猴粪便中都发现成熟虫卵。说明恒河猴可作为华支睾吸虫的终宿主。‘ 猴华支睾吸虫病早期表现主要特点有三:嗜酸性粒细胞增多症(感染后30天);慢性炎症时(感染后48天)病灶区内浆细胞显著增多;肝内胆管上皮细胞腺瘤样增生,其分泌功能亢进,经奥辛蓝染色,上皮细胞核上区呈深蓝色,提示富含酸性粘多糖。

A Preliminary Study on Clonorchis sinensis Infectionin Rhesus Monkeys

The infection of Clonorchis sinensis in two rhesus monkeys (Macaca mulatta, RH30 and RH48) was established by orally giving the metacercariae of the parasite (from Pseudorasbora parva from Sichuan) . Another monkey (RH405) served as control. The two monkeys, RH30 and RH48, were killed 30 and 48 days after infection respectively. The recovery rate of the parasite was 9.9% in RH30 and 31.59% in RH48. The worms recovered were the mature form, and mature eggs were found in the feces of the 2 monkeys 27-30 days after infecation.The pathological findings of clonorchiasis in the early stage in rhesus monkeys were eosinophilia (30 days after infection) , intralesional plasma cell infiltration (48 days after infection) , and adenomatous hyperplasia of the biliary epithelium in associatoin with an increase of acid mucin production. The significance of the last manifestation in relation to the stone formation was discussed.