RP-HPLC法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量

目的建立反相高效液相色谱法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-体积分数0.1%磷酸水溶液梯度洗脱,流速1.0 mL.min-1,检测波长为280 nm,柱温30℃。结果没食子酸、柯里拉京和短叶苏木酚的质量浓度分别在5.300～106.7 mg.L-1(r=0.999 8)、2.00～40.6 mg.L-1(r=0.999 7)、0.270～5.42 mg.L-1(r=0.999 7)内与峰面积呈良好的线性关系。没食子酸、柯里拉京和短叶苏木酚的平均加样回收率(n=9)分别为97.9%、101.7%、100.2%,RSD分别为1.8%、1.8%、2.7%。结论该分析方法准确可靠,重复性好,为更好地控制叶下珠药材的质量提供方法。

Simultaneous determination of gallic acid, corilagin and brevifolin in Phyllanthus urinaria by RP-HPLC

Objective To establish an HPLC method for simultaneous determination of gallic acid,corilagin and brevifolin in Phyllanthus urinaria.Methods The chromatographic separation was achieved on a Kromasil C18(250 mm×4.6 mm,5 μm)column with acetonitrile-0.1% phosphoric acid solution as mobile phase(gradient elution).The flow rate was 1.0 mL · min-1,the column temperature was maintained at 30 ℃,and the detective wavelength was set at 280 nm.Results The calibration curves of gallic acid,corilagin and brevifolin were linear in the range of 5.300-106.7 mg · L-1(r=0.999 8),2.00-40.6 mg · L-1(r=0.999 7),0.270-5.42 mg · L-1(r=0.999 7),respectively.The average recoveries(n=9)were 97.9%,101.7%,100.2%;and the RSDs were 1.8%,1.8%,2.7%,respectively.Conclusions The method is reliable,accurate and reproducible for quality control of P.urinaria.