山楂核化学成分的分离与鉴定

目的对山楂(Crataegus pinnatifida Bge.)核中的化学成分进行研究。方法运用硅胶﹑Seph-adex LH-20、ODS柱色谱,制备HPLC,重结晶等分离手段进行分离纯化。根据理化性质及波谱数据鉴定其结构。结果从山楂核体积分数为70%乙醇提取物中分离得到4个化合物,分别鉴定为threo-1-(4-hydroxy-3-methoxyphenyl)-2-4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(1)、erythro-1-(4-hydroxy-3-methoxyphenyl)-2-4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-pro-panediol(2)、3-(4-hydroxy-3-methoxyphenyl)-3-methoxypropane-1,2-diol(3)、2-4-(3-hydroxyprop-yl)-2-methoxyphenoxy]propane-1,3-diol(4)。结论化合物1～4为首次从山楂属植物中分离得到。

Isolation and identification of chemical constituents from seeds of Crataegus pinnatifida Bge.

Objective To study the chemical constituents from the seeds of Crataegus pinnatifida Bge. Methods The compounds were isolated by chromatography of silica gel, Sephadex LH-20, ODS column, preparative C₁₈ HPLC and recrystallization, their structures were identified on the basis of physico-chemical constant and spectral analysis. Results Four compounds were isolated from 70% ethanol extract from seeds of C. pinnatifida and identified as threo-1-(4-hydroxy-3-methoxyphenyl)-2-4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(1), erythro-1-(4-hydroxy-3-methoxyphenyl)-2-4-(3-hydroxypropyl)-2-methoxyphenoxy]-1,3-propanediol(2), 3-(4-hydroxy-3-methoxyphenyl)-3-methoxypropane-1, 2-diol (3), 2-4-(3-hydroxypropyl)-2-methoxyphenoxy]propane-1,3-diol(4). Conclusions Compounds 1-4 are firstly isolated from Crataegus genus.