| Decorin/fusin双基因修饰骨髓间充质干细胞趋化动力学及抗纤维化的研究 |
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| 引用本文： | 孟戈,张磊,陈亚进,闵军,商昌珍,吕丽虹. Decorin/fusin双基因修饰骨髓间充质干细胞趋化动力学及抗纤维化的研究[J]. 中华实验外科杂志, 2009, 27(8): 738-740. DOI: 10.3760/cma.j.issn.1001-9030.2010.06.020 |
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| 作者姓名： | 孟戈 张磊 陈亚进 闵军 商昌珍 吕丽虹 |
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| 作者单位： | 中山大学孙逸仙纪念医院肝胆胰外科,广州,510120; |
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| 基金项目： | 广东省自然学基金博士启动项目广东省科技攻关计划 |
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| 摘 要： | Objective To investigate in vitro chemotaxis dynamics and anti-fibrillar synthesis of dual-genes-modified mesenchymal stem cells ( MSCs). Methods A recombinant lentiviral-mediated chimera fusion genes was constructed using pLTR-CMV-DCN, pLTR-CMV-FIB-DCN, pLTR-CMV-FIB-Fusin plasmids and a pLVX-puro vector. Viral particles titer 1×107 cfu/ml obtained through HEK 293 cells packages were transfected into MSCs followed by puromycin selection. Transfection efficiency, gene expression were analyzed;chemotaxis,Transwell in vitro assay of fibrillar synthesis were assessed. Results Transfection efficiency 11%, after selection and purification 30%. Migrational ability and anti-fibrillar synthesis were shown under various conditions. Conclusion Dual-gene-modified mesenchymal stem cells could undergo a conformational structural change under specific microenvironment, affecting its genes expression, chemotaxis dynamics,and in vitro fibrillar synthesis.
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| 关 键 词： | 骨髓间充质干细胞 双基因修饰 趋化动力学 抗纤维合成 |
In vitro Study of chemotaxis dynamics and anti-fibrillar synthesis of Decorin/fusin dual-gene-modified mesenchymal stem cells |
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| MENG Ge,ZHANG Lei,CHEN Ya-jin,MIN Jun,SHANG Chang-zhen,LU Li-hong. In vitro Study of chemotaxis dynamics and anti-fibrillar synthesis of Decorin/fusin dual-gene-modified mesenchymal stem cells[J]. Chinese Journal of Experimental Surgery, 2009, 27(8): 738-740. DOI: 10.3760/cma.j.issn.1001-9030.2010.06.020 |
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| Authors: | MENG Ge ZHANG Lei CHEN Ya-jin MIN Jun SHANG Chang-zhen LU Li-hong |
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| Abstract: | |
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| Keywords: | Mesenchymal stem cell (MSCs)Dual-gene-modifiedChemotaxis dynamicsAnti-fibrillar synthesis |
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