下咽鳞癌患者血清比较蛋白质组学研究

Objective To detect the serum proteomic patterns in hypopharyngeal squamous cell carcinoma (HPSCC) patients. Methods The study included 58 HPSCC patients and 41 normal controls. The serum samples were analyzed for protein expression using by SELDI-TOF-MS protein chip array technology and cation-exchange (CM10) Protein array. Results There were 9 peaks differentially expressed between HPSCC patients and normal controls (P<0. 05). Using tree analysis pattern, the combination of these biomarkers could discriminate HPSCC from normal controls with a total accuracy of 90. 10%. There was no statistically significant difference in serum proteins between HPSCC patients with and without cervical lympha node metastasis ( P > 0.05 ) , and the difference between well-differentiated and poorly-differentiated cancer was quite minor. There was no any statistically significant difference between chemotherapy sensitive patients and nonsensitive patients (P>0. 05) , but 5 kinds of differentially expressed protein between radiotherapy sensitive patients and nonsensitive patients (P≤0. 05). The radiotherapy sensitive patients were segregated from nonsensitive patients with a sensitivity of 100. 00% and specificity of 83. 33%. Conclusion There are 4 peaks differentially expressed between HPSCC patients and normal controls ( P < 0.01). The serum protein at the m/z value of 6115. 74 of HPSCC radiotherapy sensitive patients is expressed higher than radiotherapy nonsensitive patients (P<0.01).

Serum comparative proteomic analysis of hypopharyngeal squamous cell carcinoma