| 高表达蛋白hSav1对人胚肾HEK293增殖能力的影响 |
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| 引用本文： | 李兆明,董硕,刘韦成,蔡少鑫,罗学来,李小兰,陶德定,严群,龚建平,胡俊波. 高表达蛋白hSav1对人胚肾HEK293增殖能力的影响[J]. 中华实验外科杂志, 2009, 27(8): 816-818. DOI: 10.3760/cma.j.issn.1001-9030.2010.06.048 |
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| 作者姓名： | 李兆明 董硕 刘韦成 蔡少鑫 罗学来 李小兰 陶德定 严群 龚建平 胡俊波 |
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| 作者单位： | 华中科技大学同济医学院附属同济医院胃肠外科/分子医学中心,武汉,430030; |
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| 基金项目： | 国家重点基础研究发展规划(973计划)国家自然科学基金湖北省自然科学基金 |
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| 摘 要： | Objective To elucidate the effects of human Salvador 1 (hSav1 ) on cell proliferation of human embryonic kidney cell line HEK293. Methods The plasmid CFP-N1-hSav1 was constructed and transfected into HEK293 cells with lipofectamine 2000. The transfection efficiency was detected by fluorescent microscopy. The effects of hSav1 on cell proliferation were measured by MTT and BrdU incorporation. Results The transfection efficiency was about 70% -80%. The MTT results showed that the inhibition rate of cell proliferation in transfected group at the 12th, 24th, 36th, and 48th h was 2% , 5% , 15% , and 23% , respectively;while that in the control group was 2% , 3% , 2% , and 2% respectively. The BrdU incorporation revealed that the BrdU incorporation rate in transfected group (12. 9 ±5. 3)% was significantly lower than in control group (27.3±3.8)% (P<0.05). Conclusion hSav1 is a newly identified protein that can cause cell proliferation inhibition in HEK293 cells.
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| 关 键 词： | 细胞增殖 人胚肾细胞 |
Effects of hSav1 on cell proliferation of human embryonic kidney cell line HEK293 |
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| LI Zhao-ming,DONG Shuo,LIU Wei-cheng,CAI Shao-xin,LUO Xue-lai,LI Xiao-Ian,TAO De-ding,YAN Qun,GONG Jian-ping,HU Jun-bo. Effects of hSav1 on cell proliferation of human embryonic kidney cell line HEK293[J]. Chinese Journal of Experimental Surgery, 2009, 27(8): 816-818. DOI: 10.3760/cma.j.issn.1001-9030.2010.06.048 |
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| Authors: | LI Zhao-ming DONG Shuo LIU Wei-cheng CAI Shao-xin LUO Xue-lai LI Xiao-Ian TAO De-ding YAN Qun GONG Jian-ping HU Jun-bo |
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| Abstract: | |
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| Keywords: | hSav1hSav1Cell proliferationHuman embryonic kidney cell |
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