| 胃癌DNA甲基化和组蛋白H3K27三甲基化基因修饰的相关性 |
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| 引用本文： | 钟克力,张丽,戴勇,潘凯,周汉新,王春友. 胃癌DNA甲基化和组蛋白H3K27三甲基化基因修饰的相关性[J]. 中华实验外科杂志, 2009, 27(8): 751-753. DOI: 10.3760/cma.j.issn.1001-9030.2010.06.024 |
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| 作者姓名： | 钟克力 张丽 戴勇 潘凯 周汉新 王春友 |
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| 作者单位： | 深圳市人民医院外科,518020;暨南大学第二临床学院广东省深圳市人民医院胃肠外科;华中科技大学同济医学院附属协和医院普外科,武汉,430022; |
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| 摘 要： | 目的 探讨DNA甲基化和组蛋白H3K27三甲基化在胃癌肿瘤细胞对多基因修饰的相关性.方法 收集我科2007年10月至2008年01月间8例胃癌患者肿瘤组织和正常胃黏膜组织,采用染色质免疫共沉淀联合芯片技术(ChIP-chip)对两种组织细胞在全基因组范围内的组蛋白H3K27三甲基化检测后挑选出4个阳性基因.随后采用染色质免疫共沉淀-实时定量聚合酶联反应(ChIP-qPCR)验证芯片结果,采用MeDIP-qPCR方法检测阳性基因的DNA甲基化水平.结果 ChIP-qPCR验证4个阳性基因结果与CpG岛芯片检测结果一致,胃癌肿瘤细胞4个基因DNA甲基化水平与正常胃壁细胞比较差异有统计学意义(P值均<0.05).结论 和正常胃黏膜组织细胞比较,胃癌肿瘤细胞多个基因DNA甲基化和组蛋白H3K27三甲基化存在显著改变,DNA甲基化和组蛋白H3K27三甲基化基因修饰存在相互作用.
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| 关 键 词： | 胃癌 组蛋白 甲基化 基因修饰 |
The correlation of gene modification between DNA methylation and histone H3 lysine 27 trimethylation in gastric cancer |
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| ZHONG Ke-li,ZHANG Li,DAI Yong,PAN Kai,ZHOU Han-xin,WANG Chun-you. The correlation of gene modification between DNA methylation and histone H3 lysine 27 trimethylation in gastric cancer[J]. Chinese Journal of Experimental Surgery, 2009, 27(8): 751-753. DOI: 10.3760/cma.j.issn.1001-9030.2010.06.024 |
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| Authors: | ZHONG Ke-li ZHANG Li DAI Yong PAN Kai ZHOU Han-xin WANG Chun-you |
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| Abstract: | Objective To study the correlation of gene modification between DNA methylation and histone H3 lysine 27 trimethylation in gastric cancer cells. Methods Tumor tissues and normal gastric mucosa in 8 cases of gastric cancer were collected from October 2007 to January 2008. After chromatin immunoprecipitatin linked to microarrays (ChIP-chip) was adopted to profile the variations in H3K27me3 in CpG island regions in tumor tissues and gastric mucosa,four genes were selected. ChIP-qPCR was used to validate the microrray results. Expression analysis by qRT-PCR was performed to confirm the correlation between H3K27me3 and gene expression. MeDIP-qPCR was used to analyze DNA methylation in four selected genes. Results The results of ChlP-qPCR were coincided well with microarray. There were significant differences in DNA methylation of four selected genes between tumor tissue and gastric mucosa (P<0.05). Conclusion There are significant differences in DNA methylation and histone H3 lysine 27 trimethylation of several genes between tumor tissue and gastric mucosa. There is a correlation of gene modification between DNA methylation and histone H3 lysine 27 trimethylation in gastric cancer |
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| Keywords: | Gastric carcinomaHistoneTrimethylationGene modification |
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