胸腺五肽对重度创伤失血性休克大鼠免疫功能的影响

目的观察胸腺五肽(TP-5)对重度创伤失血性休克大鼠免疫功能的影响。方法 54只健康雄性SD大鼠随机分为3组,即对照组(n=6)、重度创伤失血性休克模型组(模型组,n=24)、TP-5复苏组(TP-5组,n=24),在复苏后12h(T_1)、24h(T_2)、48h(T_3)、72h(T_4)各时间窗抽取静脉血,应用流式细胞仪测量T淋巴细胞亚群含量,并应用ELISA法测量肠道黏液内分泌性免疫球蛋白A(SIgA)含量。结果 模型组与TP-5组病死率均为4.17%(1/24),差异无统计学意义(P>0.05)。与对照组比较,模型组T_(1⁴)的CD_44)的CD_4⁺、CD_4+、CD_4⁺/CD_8+/CD_8⁺、SIgA含量均明显下降,CD_8+、SIgA含量均明显下降,CD_8⁺明显升高(P<0.01,P<0.05);与模型组比较,TP-5组随复苏时间延长,各项指标逐渐恢复,差异有统计学意义(P<0.01,P<0.05)。结论 早期联合应用TP-5复苏治疗对重度创伤失血性休克大鼠免疫功能有一定保护作用。

The effects of TP-5 resuscitating serious traumatic hemorrhagic shock on immune function in rats

Objective To investigate the influence of TP-S on immune function of rats with severe traumatic hemorrhagic shock.Methods 54 healthy male SD rats were randomly divided into three groups,the control group（n =6）,severe traumatic hemorrhagic shock model（model group,n =24）,TP-5 resuscitation group（TP-5 group,n =24）,in recovery after12（T1）,24（T2）,48（T3）,72（T4） each time window picked up venous blood,used flow cytometry to measure the content of T lymphocyte subsets and used ELISA to measure SIgA content within intestinal mucus.Results Model group and TP-5 group＇s mortality rate were 4.17%（1/24）,the difference was not statistically significant（P〈0.05）.Compared with the control group,model group T（1-4） of CD4^＋,CD4^＋/CD8^＋,SIgA levels were significantly decreased,CD8^＋ was significantly higher（P〈0.01,P〈0.05）;compared with the model group,TP-5 group with prolonged recovery,the index gradually recovered,significantly better than the model group（P〈0.01,P〈0.05）.Conchision Combined TP-5 early resuscitation of severe traumatic hemorrhagic shock in rats immune function have a protective effect.