苦参碱体外促进肿瘤细胞凋亡的实验研究

目的探讨苦参碱体外促进肿瘤细胞凋亡及其作用机制。方法将浓度为0.2 mg/ml、0.4 mg/ml、0.6 mg/ml的苦参碱作用于卵巢癌细胞株A21-2和骨肉瘤细胞株MG63,利用倒置相差显微镜观察不同浓度苦参碱作用后细胞的形态变化;流式细胞术(FCM)检测细胞凋亡周期、Caspase-3蛋白的表达。结果倒置相差显微镜观察表明,苦参碱能抑制MG63细胞和A21-2细胞的增殖;FCM分析发现,苦参碱与细胞作用72h后,C_0/G_1期前出现凋亡峰,与对照组比较,观察组不同浓度下凋亡率升高(P<0.01),且随浓度升高,凋亡率亦增高,差异有统计学意义(P<0.05),FCM分析显示,与对照组比较,观察组不同浓度下Caspase-3蛋白表达升高(P<0.01),且随浓度升高,Caspase-3蛋白表达亦升高,差异有统计学意义(P<005)。结论苦参碱能明显抑制A21-2细胞和MG63细胞的增殖,诱导细胞凋亡,其机制可能与促进Caspase-3蛋白的表达有关。

Experimental study of nmtrine on induction of tumor cells apoptosis in vitro

Objective To investigate the effect of matrine on induction of tumor cells apoptosis and its molecular mechanisms. Methods A21-2 and MG63 cells were treated with matrine at different concentration ：0.2 mg/ml,0.4 mg/ml, 0.6 mg/ml. Using light microscope to observe the change of morphology ; flow cytometry （FMC） method was used to detect the apoptosis cycles of cells and expression of Caspase-3 protein. Results After matrine treatment, inverted microscope observa- tion showed that the growth of cells was inhibited significantly. Flow cytometry method showed that there was a sub-Go/G1 apoptosis peak in the graph of drug-treated groups after 72h. Compared with control group, the apoptosis rate increased in obser- vation group（ P 〈 0.01 ）, and the more the concentration was , the higher the apoptosis rate was （ P 〈 0.01 ）. FCM analysis showed Caspase-3 increased in observation group than control group（ P 〈 0.01 ）, and alone with the increase of the concentration, the expression of Caspase-3 increased （ P 〈 0.05）. Conclusion Matrine can inhibit proliferation of A21-2 and MG63 cells. It＇s molecular mechanism might be related to modulating the expression of Caspase-3 protein.