黏附分子T-cadherin抑制C6胶质母细胞瘤增殖与p21表达相关

目的探讨T-cadherin分子表达抑制胶质母细胞瘤C6细胞增殖的分子机制。方法利用脂质体将pcDNA3和pcDNA3．T-cadherin表达质粒转染C6细胞，筛选出表达T-cadherin的C6克隆3、4和5以及转染空载体后不表达T-cadherin的C6克隆1和2，以Westerrl Bolt检测各克隆的p21和p27的表达；同时，以pcDNA3和pcDNA3．T-cadherin表达质粒分别转染野生型（p21＋／＋）和p21缺失突变型（p21-／-）成纤维细胞，研究T-cadherin分子介导的细胞增殖抑制是否依赖细胞周期蛋白p21的表达。结果转染pcDNA3．T-cadherin质粒后表达T-cadherin分子的C6克隆3、4和5的p21表达水平明显升高，而p27的表达与C6细胞是否表达T-cadherin无关。另外。转染pcDNA3．T-cadherin质粒的野生型表达p21的成纤维细胞的增殖显著受抑。而转染pcDNA3．T-cadherin质粒对p21缺失突变的成纤维细胞的增殖不产生抑制作用。结论T-cadherin分子抑制胶质母细胞瘤C6细胞的增殖与p21表达密切相关，且T-cadherin分子对成纤维细胞的增殖抑制作用依赖于p21的表达。

Growth suppression mediated by T-cadherin expression in C6 glioma cells is related to p21 expression

Objective To study the molecular mechanism of growth suppression mediated by T-cadherin expression in C6 glioma cells.Methods C6 cells were transfected with pcDNA3 and pCDNA3.T-cadherin using lipofectin.The T-cadherin-expressing C6 clone 3,4,5 and vector-transfected C6 clone 1,2 were screened.The expression levels of p21 and p27 levels were detected by Western blot.Wild-type fibroblast(p21+/+) and p21 mutated fibroblast(p21-/-) were transfected with pcDNA3 and pCDNA3.T-cadherin to study whether growth suppression mediated by T-cadherin expression depended on the p21 expression.Results The p21 expression levels in Tcadherin-expressing C6 clone 3,4 and 5 were significantly increased,but the p27 levels were not related to T-cadherin expression.The cell growth of wild type fibroblast(p21+/+) was significantly suppressed after transfected with pCDNA3.T-cadherin,but the cell growth of p21 mutated fibroblast(p21-/-) was affected by the transfection of pCDNA3.T-cadherin.Conclusion Growth suppression mediated by T-cadherin expression in C6 cells is closely related to the p21 expression.Moreover,the suppression mediated by T-cadherin in fibroblast depends on the p21 expression.