桑黄总三萜的提取及其体外抗脑胶质瘤U251活性

目的:优化药用真菌桑黄总三萜的超声提取工艺并测定其对脑胶质瘤U251生长的抑制作用.方法:采用正交设计试验对超声辅助提取过程中影响桑黄总三萜得率的乙醇体积分数、超声时间、超声温度和料液比等因素进行优化;采用MTT比色法测定桑黄总三萜对U251细胞株增殖的抑制率,应用倒置相差显微镜观察桑黄总三萜对肿瘤细胞形态结构的影响.结果:超声辅助提取桑黄总三萜的最佳工艺条件为70％乙醇,超声提取20 min,温度60℃,料液比1∶25.在此条件下,桑黄总三萜提取率可达9.40 mg·g-1.桑黄总三萜对脑胶质瘤U251细胞株有抑制增殖作用,且表现出浓度依赖关系,倒置相差显微镜下可见部分细胞形成凋亡小体.结论:采用超声辅助提取桑黄总三萜时间短、得率高,是一种高效、快捷的方法,桑黄总三萜可抑制脑胶质瘤U251细胞株的增殖,诱导肿瘤细胞凋亡.

Extraction of Total Triterpenoids from Phellinus igniarius and Evaluation Its Inhibitory Activity on Human Glioblastoma U251 Cells in vitro

Objective:To optimize ultrasonic-assisted extraction technology of total triterpenoids from Phellinus igniarius,and determine its inhibitory activity on human glioblastoma U251 cells in vitro.Method: Orthogonal test were implied to optimize ultrasonic-assisted extraction process with yield of total triterpenoids as index,the concentration of ethanol,ultrasonic temperature,extraction time and liquid-material ratio were optimized.MTT assay was used to determine inhibition rate of total triterpenoids on proliferation of U251 cells,Morphological changes of apoptosis of U251 cells were observed through inverted phase contrast microscope.Result: Optimum extraction conditions were determined as:the concentration of ethanol 70%,extraction time 20 min;extraction temperature 60 ℃,liquid-material ratio 1∶25,under these conditions,yield of total polyphenols reached 9.40 mg·g-1.Total triterpenoids from P.igniarius could inhibit hyperplasia of glioblastoma U251 cells in dose-dependent manner,apoptotic body formation were observed under inverted phase contrast microscope.Conclusion: Ultrasound-assisted extraction technology was an effective and rapid method for extracting total triterpenoids from P.igniarius with short time and high yield,total triterpenoids from P.igniarius could inhibit proliferation of glioblastoma U251 cells,and induced apoptosis on tumor cells.