青蒿琥酯对HeLa细胞生长的抑制作用

目的 研究青蒿琥酯对HeLa细胞生长抑制作用。方法 MTT法测定青蒿琥酯对HeLa细胞增殖抑制；用透射电镜观察青蒿琥酯对肿瘤细胞形态学影响；用流式细胞仪检测肿瘤细胞周期变化和细胞凋亡率；用免疫组化法观察肿瘤细胞端粒酶活性。结果 MTT实验表明青蒿琥酯作用HeLa细胞72h后，细胞存活率明显下降，而且随着药物浓度的增加，抑制作用增强；流式细胞仪检测青蒿琥酯可引起HeLa细胞阻滞于G1期，细胞凋亡率在一定范围内与药物浓度呈正比；透射电镜观察细胞超微结构呈现典型的凋亡特征；免疫组化检测细胞端粒酶活性降低。结论 青蒿琥酯可抑制HeLa细胞生长，诱导细胞发生凋亡，可降低细胞端粒酶活性。

Inhibiting effect of artemisinin on HeLa cell growth

Objective To investigate the inhibiting effect of artemisinin(ART)on HeLa cell growth in vitro. Methods The antiproliferation activities of ART on HeLa cells were evaluated by MTT assay.The morphological changes of ART on HeLa cells were observed under transmission electronmicroscopy.Cell cycle and apoptosis of HeLa cells influenced by ART were assessed by a flow cytometer.The telomerase activity was detected by immunohistochemistry.Results After HeLa cells were treated by ART for 72 h,the cell growth was inhibited in vitro.The inhibiting effect was increased gradually following the increase of ART concentration.The percentage of Hela cells in S phase was decreased,but increased in G_1 phase after treatment with ART.After 72 h treatment,apoptosis was induced on HeLa cells,and hTRT gene of HeLa cell was decreased obviously. Conclusion ART can inhibit the growth of human HeLa cells,induce the apoptosis,and reduce the telomerase activity.