| 葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用 |
| |
| 引用本文： | 门秀丽,赵利军,孔小燕,赵霞,李克宁,张连元. 葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用[J]. 天津医药, 2011, 39(8): 730-732. DOI: 10.3969/j.issn.0253-9896.2011.08.021 |
| |
| 作者姓名： | 门秀丽 赵利军 孔小燕 赵霞 李克宁 张连元 |
| |
| 作者单位： | 河北联合大学基础医学院病理生理教研室,唐山,063000 |
| |
| 基金项目： | 国家自然科学基金资助项目，河北省科技厅基金资助项目 |
| |
| 摘 要： | 目的:探讨葛根素联合牛磺酸对2型糖尿病大鼠胰腺的保护作用。方法:将成模的40只Wistar大鼠随机分为糖尿病组(DM组)、单用牛磺酸组(Tau组)、单用葛根素组(Pue组)和葛根素联合牛磺酸组(Pue+Tau组)。8周后,测4组大鼠糖化血红蛋白(HbA1c)、血浆葡萄糖(Glucose)、胰岛素(Insulin)、超氧化物歧化酶(SOD)、丙二醛(MDA)、活性氧(ROS)及胰腺线粒体MDA、ROS及SOD,免疫组织化学方法检测各组大鼠胰腺组织胰十二指肠同源盒基因1(PDX-1)的表达。结果:Tau组、Pue组及Pue+Tau组较DM组大鼠血浆SOD活性和血浆胰岛素水平均有显著升高,而Glucose、MDA、ROS及HbA1c呈不同程度的降低;并且3个给药组大鼠胰腺线粒体MDA和ROS含量较DM组均有不同程度的降低,而SOD活性均有显著升高,其中Pue+Tau组这种变化更加明显;免疫组织化学染色显示DM组大鼠胰岛细胞中PDX-1表达水平较低,Tau和Pue组胰岛细胞PDX-1表达上调,Pue+Tau组PDX-1表达明显上调。结论:葛根素联合牛磺酸对2型糖尿病大鼠胰腺有保护作用,其机制可能与降低氧化应激水平,上调PDX-1基因表达有关。
|
| 关 键 词： | 糖尿病,2型 牛磺酸 葛根素 胰腺 血红蛋白A,糖基化 胰岛素 免疫组织化学 模型,动物 |
Protective Effects of Puerarin Combined with Taurine on Pancreas of Rats with Type 2 Diabetes |
| |
| MEN Xiuli,ZHAO Lijun,KONG Xiaoyan,ZHAO Xia,LI Kening,ZHANG Lianyuan. Protective Effects of Puerarin Combined with Taurine on Pancreas of Rats with Type 2 Diabetes[J]. Tianjin Medical Journal, 2011, 39(8): 730-732. DOI: 10.3969/j.issn.0253-9896.2011.08.021 |
| |
| Authors: | MEN Xiuli ZHAO Lijun KONG Xiaoyan ZHAO Xia LI Kening ZHANG Lianyuan |
| |
| Affiliation: | MEN Xiuli,ZHAO Lijun,KONG Xiaoyan,ZHAO Xia,LI Kening,ZHANG Lianyuan Department of Pathophysiology,North China Coal Medical University,Tangshan 063000,China |
| |
| Abstract: | Objective:To investigate the protective effects of puerarin combined with taurine on pancreas of rats with type 2 diabetes.Methods:Forty Wistar rats with diabetes mellitus were randomly divided into diabetes mellitus group(DM),taurine treatment group(Tau),puerarin treatment group(Pue) and puerarin combined with taurine treatment group(Pue+Tau).After 8-week treatment,the serum levels of glycosylated hemoglobin(HbA1c),glucose,insulin,malondialdehyde(MDA),superoxide dismutase(SOD) and reactive oxygen species(R... |
| |
| Keywords: | diabetes mellitus type 2 TAURINE PUERARIN pancreas hemoglobin A glycosylated insulin immunohistochemistry models animal |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《天津医药》浏览原始摘要信息 |
|
点击此处可从《天津医药》下载全文 |
|
