| 卵母细胞线粒体DNA(mtDNA)拷贝数在卵巢储备功能下降中的研究 |
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| 引用本文： | 赵红翠,李艳萍. 卵母细胞线粒体DNA(mtDNA)拷贝数在卵巢储备功能下降中的研究[J]. 生殖与避孕, 2011, 31(1): 53-57 |
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| 作者姓名： | 赵红翠 李艳萍 |
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| 作者单位： | 1. 中南大学湘雅医院妇产科生殖医学中心,长沙,410008;解放军306医院生殖医学中心,北京,100101
2. 中南大学湘雅医院妇产科生殖医学中心,长沙,410008 |
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| 基金项目： | 湖南省科厅科技计划一般项目 |
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| 摘 要： | 目的:初步探讨卵母细胞线粒体DNA(mtDNA)拷贝数对卵巢储备功能下降患者生育潜能的影响。方法:选取IVF-ET治疗的不孕症患者80例,按照卵巢储备功能状态分为卵巢储备功能下降(DOR)组和卵巢储备功能正常(NOR)组,胚胎移植日收集患者自愿捐赠的未受精的废弃MII期卵母细胞,Real-timePCR方法定量检测比较卵母细胞mtDNA拷贝数。结果:共收集到废弃的MII期卵母细胞110个,其中DOR组46个,NOR组64个。DOR组卵母细胞mtDNA拷贝数下降(9.96×104±5.21×104vs1.77×105±7.27×104),组间具有显著性差异(P<0.05)。结论:卵巢储备功能下降患者卵母细胞mtDNA拷贝数减少,可能与其卵母细胞发育潜能下降相关。
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| 关 键 词： | 卵母细胞 卵巢储备功能 mtDNA拷贝数 实时荧光定量PCR |
Study on mitochondrial DNA content in unfertilized oocytes from diminished ovarian reserve patients |
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| Hong-cui ZHAO,Yan-ping LI. Study on mitochondrial DNA content in unfertilized oocytes from diminished ovarian reserve patients[J]. Reproduction and Contraception, 2011, 31(1): 53-57 |
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| Authors: | Hong-cui ZHAO Yan-ping LI |
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| Affiliation: | 1(1.Reproductive Medical Center,Xiangya Hsopital,Central South University,Changsha,410008)(2.Department of Reproductive Medicine,the 306th Hospital of P.L.A,Beijing,100101) |
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| Abstract: | Objective:To explore the effect of mtDNA content on diminished ovarian reserve patients oocytes reproductive potential.Methods:Eighty infertility patients were enrolled.According to the ovarian reserve,they were divided into diminished ovarian reserve(DOR) group and normal ovarian reserve(NOR) group.Unfertilized abandonable MII oocytes were collected on embryo-transfer day.mtDNA content was quantified by real-time PCR.The two groups mtDNA was compared.Results:A total of 110 voluntarily donated unfertilized abandonable MII oocytes were collected,46 in DOR group,and 64 in NOR group.DOR group showed significantly lower mtDNA content(9.96×104±5.21×104 vs 1.77×105±7.27×104,P<0.05).Conclusion:The low mtDNA content in DOR patients oocyte may be related to their decreased oocyte developmental potential. |
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| Keywords: | oocyte ovarian reserve mtDNA content real-time PCR |
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