| 一种胚胎体外培养的新方法:滴中孔培养法 |
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| 引用本文： | 冯卫国,潘智芳,刘晓影,于文静,赵春玲,高志芹.一种胚胎体外培养的新方法:滴中孔培养法[J].生殖与避孕,2010,30(11):721-725. |
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| 作者姓名： | 冯卫国 潘智芳 刘晓影 于文静 赵春玲 高志芹 |
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| 作者单位： | 潍坊医学院,潍坊,261053 |
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| 基金项目： | 山东省自然科学基金资助项目 |
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| 摘 要： | 目的:建立并优化一种胚胎体外培养的新方法——滴中孔培养法。方法:用圆头玻璃管在培养皿上烫出多个圆孔,作为滴中孔培养的专用平皿;收集牛卵丘-卵母细胞复合体(COCs)并在滴中孔培养专用平皿中进行体外成熟(IVM)、体外受精(IVF)和体外培养(IVC)。结果:①滴中孔培养法与群卵共培养的效果相似,而单卵培养明显降低了卵母细胞的发育能力(P<0.05)。②培养滴体积和培养密度对卵母细胞核成熟没有明显的影响,但显著影响胚胎的发育能力。在培养密度不变的情况下,培养滴体积太小显著削弱了胚胎的发育能力,而培养密度过小或者过大也削弱胚胎的发育能力(P<0.05)。结论:滴中孔培养法可以达到群卵共培养的效果。
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| 关 键 词： | 滴中孔培养法 培养滴体积 培养密度 卵母细胞发育能力 牛 |
A New Method for Embryo Culture in vitro: the Well in the Drop(WID)System |
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| Wai-guo FENG,Zhi-fang PAN,Xiao-ying LIU,Wen-jing YU,Chun-ling ZHAO,Zhi-qin GAO.A New Method for Embryo Culture in vitro: the Well in the Drop(WID)System[J].Reproduction and Contraception,2010,30(11):721-725. |
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| Authors: | Wai-guo FENG Zhi-fang PAN Xiao-ying LIU Wen-jing YU Chun-ling ZHAO Zhi-qin GAO |
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| Institution: | Wai-guo FENG,Zhi-fang PAN,Xiao-ying LIU,Wen-jing YU,Chun-ling ZHAO,Zhi-qin GAO (Weifang Medical University,Weifang,261053) |
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| Abstract: | Objective: To establish and optimize a new method of embryo culture in vitro: the well in the drop (WID) system.Methods: The glass tube was used to make some round hole on the dish,to make a special dish for WID system;Bovine COCs and cultured in WID system in vitro maturation (IVM),in vitro fertilization (IVF) and in vitro culture (IVC).Results: 1) The WID culture system works as efficient as group culture,whereas the individual culture impaired the oocyte developmental competence significantly (P<0.05).2)... |
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| Keywords: | WID medium volume culture density oocyte developmental competence bovine |
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