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天津地区临床分离结核分枝杆菌分型的初步研究
引用本文:柴利泉,李丽,白大鹏,张立,邵世峰,吴琦,陆伟,孙照刚,李传友,李卫民,戴宗甲.天津地区临床分离结核分枝杆菌分型的初步研究[J].中华流行病学杂志,2007,28(8):785-788.
作者姓名:柴利泉  李丽  白大鹏  张立  邵世峰  吴琦  陆伟  孙照刚  李传友  李卫民  戴宗甲
作者单位:1. 300350,天津市海河医院结核病科
2. 北京市结核病胸部肿瘤研究所
基金项目:北京市自然科学基金资助项目(5062016);北京市优秀人才培养专项经费资助项目
摘    要:目的探讨天津地区结核分枝杆菌临床分离株分子流行病学特征。方法连续收集天津市海河医院2005年8月16日11月25日就诊患者痰培养阳性的结核分枝杆菌100株,采用间隔区寡核苷酸分型(spoligotyping)和多位点可变串联重复序列(VNTR)两种方法进行基因分型,并运用软件对二者的结果进行分析。依据北京分化支的定义,运用多重和实时定量PCR方法将其区分为W菌/典型北京家族菌株和非典型北京菌株,Χ^2检验分析两种亚群与患者年龄和耐药性之间的联系。结果排除污染菌株,共对96株结核分枝杆菌临床分离株进行两种方法的基因分型,spoligotyping结果91.7%为北京基因型(含3株类北京基因型)结核分枝杆菌(88/96)。VNTR分型可将北京基因型分为60种基因型。在北京分化支结核分枝杆菌中,W菌/典型北京家族菌株占93.2%(82/88)。两种北京分化支亚群与患者年龄及耐药性之间差异无统计学意义(P〉0.05)。结论天津地区结核病患者临床分离的结核分枝杆菌中,北京基因型呈现较为明显的优势。VNTR的分辨率明显高于spoligotyping。北京分化支的两种亚群在天津地区临床结核病患者中均有流行,但以W菌/典型北京家族菌株为主。

关 键 词:结核分枝杆菌  北京基因型  北京分化支  W菌/典型北京家族菌株  非典型北京菌株
收稿时间:2007/1/25 0:00:00
修稿时间:2007-01-25

Study on the genotype of Mycobacterium tuberculosis isolates from hospitals in Tianjin
CHAI Li-quan,LI Wei-min,LI Li,DAI Zong-ji,BAI Da-peng,ZHANG Li,SHAO Shi-feng,WU Qi,LU Wei,SUN Zhao-gang and LI Chuan-you.Study on the genotype of Mycobacterium tuberculosis isolates from hospitals in Tianjin[J].Chinese Journal of Epidemiology,2007,28(8):785-788.
Authors:CHAI Li-quan  LI Wei-min  LI Li  DAI Zong-ji  BAI Da-peng  ZHANG Li  SHAO Shi-feng  WU Qi  LU Wei  SUN Zhao-gang and LI Chuan-you
Institution:Department Tuberculosis , Haibe Hospital, Tianjin 300350 ,China
Abstract:OBJECTIVE: To explore the characteristics on molecular epidemiology of Mycobacterium tuberculosis isolates from hospitals in Tianjin area. METHODS: One hundred M. tuberculosis isolated strains were collected in succession from August 16th-December 25th, 2005 in Tianjin Haihe Hospital and genotyped by spoligotyping and multiple locus variable number-tandem repeat(VNTR). Data was analyzed by cluster software. Based on the concept of Beijing lineage, it was determinate two sub-groups: atypical Beijing strains and W strain/typical family strains by multiplex and real-time PCR. The associations of subgroups with drug resistance and age were assessed by the chi2 test. RESULTS: 96 M. tuberculosis strains were genotyped in which 91.7% (88/96) strains belonged to Beijing genotype (including 3 Beijing-like strains) by spoligtyping. VNTR typing could differentiate 60 genotypes among the 88 Beijing genotype strains. 93.2% of the Beijing lineage M. tuberculosis strains of this study belonged to W strain/typical Beijing family strains (82/88). No statistically significant differences were observed in the proportions of the two sub-groups in patients of different age, or drug resistance (P > 0.05). CONCLUSION: The M. tuberculosis Beijing genotype strains were dominated on tuberculosis hospital patients of Tianjin area. The discriminatory power of VNTR typing was higher than that of spoligtyping. The two sub-groups of Beijing lineage had been prevalent in Tianjin, however W strain/typical Beijing family strains were of preponderance.
Keywords:Mycobacterium tuberculosis  Beijing genotype  Beijing lineage  W strain/typical Beijing family strains  Atypical Beijing strains
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