大鼠枕大池二次注血蛛网膜下腔出血延迟性脑血管痉挛模型的建立

目的建立可靠的大鼠蛛网膜下腔出血后迟发性脑血管痉挛模型。方法对36只SD大鼠随机分为蛛网膜下腔出血(SAH)和注生理盐水对照(NS)两组,采用枕大池二次注血的方法建立SAH模型,NS组同法注等量的NS;在首次注血或注水后35、、7天,每组各灌注处死6只大鼠,取其基底动脉比较基底动脉的内径周长和血管壁厚度。结果与NS组相比,SAH组注血后3、5、7天,基底动脉的内径周长和血管壁厚度均有显著性差异,脑血管痉挛在第7天达到高峰。结论大鼠枕大池二次注血法是可靠的SAH后迟发性脑血管痉挛模型制作方法。

Establishing models of subarachnoid hemorrhage in rats by double injections of blood into cistern magna

Objective To establish a reliable models of delayed cerebral vasospasm(DCV) after subarachnoid hemorrhage(SAH) in rats.Methods 36 Sprague-Dawley rats were randomly divided equally into SAH and saline control(NS) groups.SAH were developed with two times injections of 0.3 ml arterial blood into cisterna magna,and for group NS 0.3 ml saline injected.The basilar arteries of 6 rats were taken for measurements of luminal inner perimeter and wall thickness at 3,5,7 days of experiments for each group respectively.The luminal inner perimeter and wall thickness were measured by Motic image system.Results Compared with the group NS,luminal inner perimeter of basilar arteries were significantly reduced for group SAH,and wall thickness significantly increased at 3,5,7 days of experiments.There were significant differences betweem two groups.Maximum narrowing of basilar arteries at 7 days.Conclusion Two times injections of arterial blood into cisterna magna is a reliable method for making DCV after SAH in rats.