| 共轭方向法在HPLC流动相条件优化中的应用——麻黄碱、伪麻黄碱的分离 |
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| 引用本文： | 梁宏晞,于如嘏,杨清华,倪坤仪. 共轭方向法在HPLC流动相条件优化中的应用——麻黄碱、伪麻黄碱的分离[J]. 药学学报, 1991, 26(1): 49-52 |
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| 作者姓名： | 梁宏晞 于如嘏 杨清华 倪坤仪 |
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| 作者单位： | 中国药科大学，中国药科大学，中国药科大学，中国药科大学 南京 210009，南京 210009，南京 210009，南京 210009 |
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| 摘 要： | 麻黄碱与伪麻黄碱是中药麻黄的主要有效成分,也是一对化学物理性质很相似的差向异构体。二者的结构只是构象不同,用色谱方法进行分离有一定的难度。目前已有在苯基和腈基键合固定相上进行HPLC分离的报道(1,2)。本文成功地引入共轭方向法在μBondapak C18色谱柱上对流动相组成进行了条件优化,优化后的色谱条件下麻黄碱与伪麻黄碱的分离情况令人满意。
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| 关 键 词： | 共轭方向法 优化 高效液相色谱法 麻黄碱 伪麻黄碱 |
| 收稿时间： | 1989-09-23 |
USE OF THE POWELL METHOD TO OPTIMIZE THE COMPOSITION OF HPLC MOBILE PHASE THE SEPARATION OF EPHEDRINE AND PSEUDOEPHEDRINE |
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| HX Liang,RG Yu,QH Yang and KY Ni. USE OF THE POWELL METHOD TO OPTIMIZE THE COMPOSITION OF HPLC MOBILE PHASE THE SEPARATION OF EPHEDRINE AND PSEUDOEPHEDRINE[J]. Acta pharmaceutica Sinica, 1991, 26(1): 49-52 |
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| Authors: | HX Liang RG Yu QH Yang KY Ni |
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| Affiliation: | China Pharmaceutical University, Nanjing. |
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| Abstract: | The isomers ephedrine and pseudoephedrine are difficult to be separated by HPLC. In this paper, the Powell method was successfully introduced to optimize the composition of mobile phase in HPLC. On a mu Bondapak C18 column (8-10 microns, 3.9 mm x 30 cm), KH2PO4 solution methanol mobile phase system was optimized for separating ephedrine and pseudoephedrine by the Powell method. The optimized composition of the mobile phase was found as: 0.01 mol/L KH2PO4 -MeOH = 94: 6 (V/V). The Powell method was carried out in a process illustrated by tables and figures and 0.95 peak separation function was achieved. |
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| Keywords: | Powell method Optimization HPLC Ephedrine Pseudoephedrine |
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