龟鹿二仙胶抵抗化疗小鼠脾T淋巴细胞凋亡的实验研究

目的 探讨龟鹿二仙胶对化疗小鼠的抗凋亡作用及作用机理。方法 将荷瘤昆明种小鼠72只随机分为环磷酰胺（CTX）加龟鹿二仙胶高、中、低3个剂量组，CTX组，单纯中药组，生理盐水组6组。给药9天后处死小鼠取脾组织，采用流式细胞仪检测T淋巴细胞凋亡；RT PCR法检测bcl 2、Caspase 3 mRNA的表达。结果 CTX组荧光素标记的膜联蛋白阳性（FITC+Annexin V）/碘化丙啶(PI)染色阴性比例（FITC+/ PI－）T细胞较生理盐水组增高，差异有统计学意义（P<0.05）；CTX加用龟鹿二仙胶的3个剂量组FITC+/ PI－T细胞比例较CTX组明显降低（P<0.05）。CTX组bcl 2 mRNA表达量较生理盐水组减少（P<0.05）；CTX加用龟鹿二仙胶的3个剂量组其表达量较CTX组明显升高（P<0.05）。Caspase-3 mRNA表达量CTX组升高（P<0.05）；CTX加用龟鹿二仙胶的3个剂量组表达量较CTX组明显减少。结论 龟鹿二仙胶能有效抑制化疗小鼠淋巴细胞凋亡。上调bcl-2 mRNA表达、下调Caspase-3mRNA表达可能是其作用机理之一。

Effect of Guilu Erxianjiao in Suppressing Splenic T-Lymphocyte Apoptosis in Mice Undergoing Chemotherapy

OBJECTIVE: To investigate the effect of Guilu Erxianjiao (GLEXJ) in suppressing chemotherapy induced cell apoptosis in mice and to discuss its possible acting mechanism. METHODS: Seventy-two tumor-bearing Kunming mice were randomly divided into 6 groups, Group A, treated with normal saline; Group B treated with cyclophosphamide (CTX), Group C treated with Chinese herbal medicine, and Group D-F, the three combined treated group received CTX plus high, moderate and low dose GLEXJ. All mice were sacrificed after 9-day medication, their splenic tissues were taken for determining T-lymphocyte apoptosis with flow cytometer, the expression of bcl-2 mRNA and Caspase-3mRNA detected by RT-PCR. RESULTS: As compared with Group A, FITC+/PI- cell proportion of spleen T-lymphocyte cells and Caspase-3mRNA expression were higher, while bcl-2 mRNA expression was lower in Group B; as compared with Group B, the former two indexes were lower and the latter was higher in the three combined treated groups (all P <0.05). CONCLUSION: GLEXJ could efficiently suppress the splenic T-lymphocyte apoptosis in tumor bearing mice undergoing chemotherapy, one of its mechanisms may be through up-regulating of bcl-2 mRNA expression and down-regulating of Caspase-3mRNA expression.