石榴皮水提物治疗溃疡性结肠炎模型大鼠的实验研究

目的：观察石榴皮水提物对慢性溃疡性结肠炎模型大鼠的治疗作用。方法：将80只SD大鼠随机分为正常对照组、模型组、柳氮磺吡啶（SASP）组、石榴皮水提物低、中、高剂量组（200、400、800 mg/kg）。用2,4-二硝基氯苯（DNCB）复合乙酸法建立大鼠溃疡性结肠炎模型。连续给药4周后麻醉处死动物,观察大鼠结肠大体形态的变化并进行结肠黏膜损伤指数（CMDI）评分,并测定大鼠结肠重量、肠重指数、组织髓过氧化物酶（MPO）活力、白介素1β（IL-1β）、肿瘤坏死因子α（TNF-α）及丙二醛（MDA）含量。结果：与模型组比较,石榴皮水提物中、高剂量组及SASP组大鼠腹泻症状明显缓解。IL-1β、TNF-α、MDA含量和MPO活力显著降低（P〈0.05）;病理学检查或尸检可见结肠组织溃疡面积明显缩小,水肿缓解,组织坏死减轻,未见肠壁增厚。石榴皮水提物高、中剂量组治疗效果明显优于SASP组（P〈0.05）。结论：石榴皮水提物能显著缓解DNCB复合乙酸法所致慢性溃疡性结肠炎的症状,治疗作用明显。

Experimental study of aqueous extract of Pericarpium granati for treating ulcerative colitis of rats

Objective： To observe the therapeutic effect of aqueous extract of Pericarpiurn granati （AEOPG） on chronic ulcerative colitis（UC） model rats. Methods： Eighty SD rats were randomly divided into control group, model group, sulfasalazine（SASP） group, low-dose group（200 mg/kg）, middle-dose group（400 mg/kg）, and high-dose group（800 mg/kg） of AEOPG. UC model was established by 2,4-dinitrochlorobenzene（DNCB） and acetic acid method. After 4 weeks of intragastric administration of AEOPG, the rats were sacrificed with anesthesia, and the gross morphology of colon of the rats was observed and colonic mucosal damage index（CMDI） of the rats was rated. The weight of the colon, the intestinal weight index, the activity of intestinal myeloperoxidase（MPO）, and the concentrations of interleukin-1β（IL-1β） and tumor necrosis factor α （TNF-α） in serum and malondialdehyde（MDA） in colon were determined. Results： Compared with the model group, the diarrheic symptoms of the UC rats in SASP group, middle dose and high dose AEOPG were significantly relieved, and the activity of MPO, the concentrations of MDA, IL-1β,TNF-α were significantly decreased（P〈0.05）. Pathological examination or autopsy showed that the area of colonic ulcer was significantly reduced, colonic edema was alleviated, the necrosis of tissue was reduced, and colonic wall was not thickened. The therapeutic effects of middle- and high-dose AEOPG groups were obviously superior to that of SASP group （P〈0.05）. Conclusion： AEOPG can significantly relieve the symptoms of UC rats induced by DNCB with acetic acid.