自制扩散匣的免疫隔离作用及匣内异基因瘤细胞的在体生长观察

目的 观察自制细胞扩散匣的免疫隔离作用及匣内异基因瘤细胞的在体生长情况。方法 通过PEG介导制备SP2/0-BALB／c小鼠脾细胞杂交细胞。注入BALB／c小鼠和C57BL／6(B6)小鼠腹腔，观察腹水产生和瘤体形成，了解杂交细胞MHC抗原携带情况。再将含杂交细胞的微孔膜扩散匣分别植入BALB／c和B6小鼠腹腔，观察匣内细胞生存情况。结果 腹腔注射杂交细胞后2个月内，BALB／c小鼠产生血性腹水，或形成瘤体，B6小鼠未见腹水产生和瘤体形成。腹腔植入杂交瘤细胞扩散匣后30d和101d，两组动物体内扩散匣均被大网膜或肠系膜包裹，外壁可见新生血管，匣内形成瘤体，但不能充满整个匣腔。结论 SP2／0细胞与BALB／c小鼠脾细胞融合后可转变为携带BALB／c品系MHC抗原的永生细胞，在异基因宿主(B6小鼠)体内不能存活。自制微孔膜扩散匣能有效起到免疫隔离作用。微孔膜外表面能够形成新的营养血管，支持匣内细胞长期生存与增殖。

Immune isolation of diffusion chamber for hybridoma

Objective To determine the effect of diffusion chamber for immune isolation and the proliferation of allogenic hybridoma within diffusion chamber in mice. Methods The hybridoma cell strain was established from SP2/0 cells and the splenocytes of BALB/c mouse by PEG mediate technique. The cells were injected peritoneally into BALB/c ( n =5) and C57 BL/6 (B6, n =5) mice. Diffusion chambers with microporous membrane containing hybridoma cells were implanted peritoneally into 5 BALB/c and 5 B6 mice. Results Within two months after peritoneal injection of the cell strain, four BALB/c mice developed bloody ascites and one developed abdominal tumor, but the B6 mice developed neither ascites nor tumor. After 30 and 101 days of the chamber implantation, the chambers were coated by omentum or mesenterium in all the animals of both strain groups and freshly formed blood vessels to the chamber membrane could be observed. Within the chambers, tumor developed, but the tumor could not fully fill the inner space of the chamber during the whole observation period. Conclusion The hybridoma cells established from SP2/0 cells and the splenocytes of BALB/c mouse may carry BALB/c MHC antigen. The diffusion chambers can effectively isolate immune rejection. Freshly formed blood vessels to the chamber membrane can support the cells within the chambers.