Phenotypic differences of CD4+ T cells in response to red blood cell immunization in transfused sickle cell disease patients |
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Authors: | Benoît Vingert Marie Tamagne Anoosha Habibi Sadaf Pakdaman Julie Ripa Rahma Elayeb Frédéric Galacteros Philippe Bierling Hélène Ansart‐Pirenne Pablo Bartolucci France Noizat‐Pirenne |
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Institution: | 1. Etablissement Fran?ais du Sang, Créteil, France;2. Inserm, U955, Equipe 2, Créteil, France;3. AP‐HP, H?pital H. Mondor—A, Chenevier, Service Maladies Génétiques du Globule Rouge, Créteil, France;4. Faculté de médecine, Université Paris Est, Créteil, France |
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Abstract: | Alloimmunization against red blood cells (RBCs) is the main immunological risk associated with transfusion in patients with sickle cell disease (SCD). However, about 50–70% of SCD patients never get immunized despite frequent transfusion. In murine models, CD4+ T cells play a key role in RBC alloimmunization. We therefore explored and compared the CD4+ T‐cell phenotypes and functions between a group of SCD patients (n = 11) who never became immunized despite a high transfusion regimen and a group of SCD patients (n = 10) who had become immunized (at least against Kidd antigen b) after a low transfusion regimen. We studied markers of CD4+ T‐cell function, including TLR, that directly control lymphocyte function, and their spontaneous cytokine production. We also tested responders for the cytokine profile in response to Kidd antigen b peptides. Low TLR2/TLR3 expression and, unexpectedly, strong expression of CD40 on CD4+ T cells were associated with the nonresponder status, whereas spontaneous expression of IL‐10 by CD4+ T cells and weak Tbet expression were associated with the responder status. A Th17 profile was predominant in responders when stimulated by Jbk. These findings implicate CD4+ T cells in alloimmunization in humans and suggest that they may be exploited to differentiate responders from nonresponders. |
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Keywords: | Alloimmunization CD4+ T  cells Jkb Sickle cell disease Th17 |
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