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| PLGA与明胶膜支架材料对神经干细胞生长、分化影响的体外研究 | |
| 引用本文： | 谭太贵,马景鑑,张文治,苏心,王立凯,冯喜增. PLGA与明胶膜支架材料对神经干细胞生长、分化影响的体外研究[J]. 天津医药, 2006, 34(9): 640-641,673 |
| 作者姓名： | 谭太贵 马景鑑 张文治 苏心 王立凯 冯喜增 |
| 作者单位： | 1. 30052,天津医科大学总医院神经外科 2. 天津市神经外科研究所神经细胞研究室 3. 南开大学生命科学院生物活性材料教育部重点实验室 |
| 摘 要： | 目的：探讨PLGA作为中枢神经组织工程支架材料的可行性。方法：从胎龄14～16d的Wistar大鼠分离培养获得神经干细胞，以10^8/L密度种植于PLGA膜、明胶膜和PLGA／明胶膜表面，置37℃、5％CO2培养箱中共培养，观察神经干细胞在PLGA膜、明胶膜和PLGA／明胶膜表面的生长、分化和PLGA的降解情况。结果：培养获得的神经干细胞表现具有自我更新和多相分化能力，能分化为神经元和胶质细胞。在观察期内PLGA膜无降解，神经干细胞在PLGA膜表面无贴附，但可在PLGA／明胶膜和明胶膜表面贴附、生长和分化。结论：PLGA可作为中枢神经组织工程支架材料。 |
| 关 键 词： | 聚乙醇酸 乳酸 明胶 组织工程 干细胞 中枢神经,系统 大鼠 |
| 收稿时间： | 2005-09-01 |
| 修稿时间： | 2005-09-012006-05-27 |
Vitro Study on the Effects of PLGA and Gelatin Films Scaffold Materials on Neural Stem Cells'''' Growth and Differentiation |
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| TAN Taigui,MA Jingjian,ZHANG Wenzhi,SU Xin,WANG Likai,FENG Xizeng. Vitro Study on the Effects of PLGA and Gelatin Films Scaffold Materials on Neural Stem Cells'''' Growth and Differentiation[J]. Tianjin Medical Journal, 2006, 34(9): 640-641,673 | |
| Authors: | TAN Taigui MA Jingjian ZHANG Wenzhi SU Xin WANG Likai FENG Xizeng |
| Affiliation: | Department of Neurosurgery, Tianjin Medical University General Hospital, Tianjin 300052, China |
| Abstract: | Objective: To explore the possibility of PLGA as a scaffold biomaterial for central nervous system regeneration. Methods: Neural stem cells were respectively seeded on the surfaces of PLGA, gelatin and PLGA/gelatin films at a density of 108cells/L. The growth and differentiation of neural stem cells and the degradation of PLGA were observed. Results: During the follow-up, PLGA didn't degrade, no neural stem cells attached on the surface of PLGA films, but the cells attached, grew and differentiated on the surface of PLGA/gelatin and gelatin films. Conclusion: PLGA can be used for an ideal scaffold biomaterial for central nervous system regeneration. |
| Keywords: | Polygly coli acid lactic acid gelatin tissue engineering stem cells central nervous system rats |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |