实验性玻璃体腔内高氧对视网膜超微结构的影响

目的 通过研究玻璃体腔内高氧对视网膜超微结构的影响，观察氧对视网膜组织的毒性作用，为实现临床应用玻璃体腔内的氧疗提供基础实验依据。方法：以2个月龄猪为实验动物，模拟临床常规玻璃体切割手术后眼内充填混合气体，在混合气体中提高氧比例达40％，设置眼内充填常规混合气体组和空白对照组做为实验对照组，持续数天后观察视网膜全层超微结构的改变。结果 眼内高氧组7d即可引起视网膜神经节细胞核膜的明显溶解，双层膜结构消失，且导致胞浆内线粒体肿胀，双层膜结构模糊，嵴排列出现紊乱。结论 玻璃体腔内的氧含量增加到一定程度（40％）时，则可引起视网膜组织的氧化损害，具有一定的氧毒性。

Effects of experimental intravitreous hyper oxygen on the retinal ultrastructure

Objective To study the toxic effects of intravitreous hyper oxygen on retina by observing the change of the pig retinal ultrastructure when the oxygen proportion in intravitreous mixed gases is increased. To provide experimental basis for clinical apply of intravitreous treatment of hyper oxygen. Methods Experimental animal: 2 months pigs. Pouring mixed gases into vitreous after clinical conventinal vitrectomy. Increasing the proportion of oxygen in mixed gases to 40% contrasting to rule mixed gases group and blank control group.Then observing the change of the retinal ultrastructure in every group after 7 days. Results After 7 days,there was a obvious dissolution of nucleus membrane and arrange disorder on plastosome ridge of retinal gangliocyte in hyper oxygen group. Conclusion Increasing the proportion of intravitreous oxygen to 40% can result some damaged change of the retinal ultrastructure.So there is some toxic effect for intravitreous hyper oxygen to retina.