脑源性神经营养因子G196A基因多态性与抑郁症及认知功能的关系

目的:探索脑源性神经营养因子(BDNF)G196A基因多态性与中国汉族人群抑郁症及其认知功能的关系。方法:采用病例对照研究方法,以153例抑郁症患者及180名正常对照人群为研究对象,采用PCR-RFLP技术检测BDNF G196A基因多态性,采用连线测验A和B、言语流畅性测验、威斯康辛卡片分类测验-改良版(M-WCST)、汉诺塔测验评定患者的认知功能。比较抑郁症患者与正常对照组BDNF基因型及等位基因频率的差异;比较携带不同等位基因或基因型的抑郁症患者认知功能的差异。结果:抑郁症患者BDNF基因A等位基因的频率(60.1%)高于对照组(52.2%),比较差异有统计学意义(P<0.05)。携带BDNF 196A等位基因的抑郁症患者的各项认知功能测验的成绩与没有携带A等位基因者比较差异无统计学意义(P>0.05)。按基因型分类比较抑郁症患者的认知功能,携带A/A型患者的WSCT分类数低于其它两种基因型携带者、WSCT持续错误数高于其他两种基因型携带者(P<0.05)。结论:BDNF 196A等位基因是抑郁症发病的危险因素,携带A/A基因型的抑郁症患者执行功能损害更严重。

The correlation between brain-derived neurotrophic factor gene G196A polymorphism and depression or cognitive function

Objective： To investigate the correlation between brain-derived neurotrophic factor （BDNF） G196A gene polymorphism and depression or cognitive function in Chinese Han population. Methods： Correlation study was performed in 153 patients with depression and 180 health individuals in Guangzhou, China. The G196A polymorphism of BDNF gene promoter was analyzed by polymerase chain reaction-restriction fragment length poly- morphism （PCR-RFLP）. The cognitive function were assessed by Trail making test, verbal fluency, modified Wis- consin card sorting test （M-WCST）, Tower of Hanoi test. Results： The frequency of BDNF gent 196A allele in depression group （60. 1% ） was significantly higher than that in control group （52. 2%, P 〈 0. 05 ）. The results of cognitive function test in BDNF196A allele carriers were all not significantly different from those in non-A allele carriers （P 〉 0.05 ）. The depressive patients with A / A genotype had significantly more categories in WSCT and less continuing errors than those in the other two genotype carriers （P 〈 0. 05）. Conclusion： The A allele of BD-NF gene is a risk factor of depression. The depressive patients with A / A genotype performed worse executive func- tion.