电针对全脑缺血再灌注损伤高龄大鼠大脑皮质P53蛋白表达的影响

目的 :观察电针对全脑缺血再灌注损伤后高龄大鼠大脑皮质P5 3蛋白表达的影响。方法 :采用改良的Pulsinelli 4 血管阻断 ( 4 VO)方法制备SD高龄大鼠全脑缺血再灌注损伤模型 ,将大鼠随机分为假手术组、缺血再灌注组、缺血再灌注 +电针组。假手术组动物仅烧灼双侧翼小孔内椎动脉 ,但不夹闭双侧颈总动脉 ,电针组在缺血再灌注后立即予电针治疗 ,以后每天 1次。在缺血再灌注 3天 ( 72hr)后将大鼠处死 ,进行免疫组织化学染色。结果 :缺血再灌注组大鼠大脑皮质P5 3蛋白免疫阳性细胞数明显高于假手术组 (P <0 <0 5 )。缺血再灌注 +电针组大鼠大脑皮质P5 3蛋白免疫阳性细胞数明显低于缺血再灌注组 (P <0 <0 5 )。结论 :电针可明显减少全脑缺血再灌注损伤后高龄大鼠大脑皮质P5 3蛋白的表达 ,这可能是电针抗脑缺血后神经元凋亡的途径之一

Effect of EA on Cerebral Ischemia-reperfusion Induced Changes of P53 Protein Expression in Cerebral Cortex of Elder Rats

Objective:To observe the effect of electroacupuncture( EA) on P53 p rotein expression in rats with cerebral ischemia-reperfusion injury and to anal yze the mechanisms of EA in reducing neuronal apoptosis. Method: 36 S D rats were randomly and evenly divided into sham-operation group,model group an d EA group.Cerebral ischemia-reperfusion model was established by using modifie d (Pulsinelli's)4-vessel occlusion method. Bilateral"Zusanli"(ST 36) were pu nctured and stimulated with an EA therapeutic apparatus (100 Hz ,3.5 mA and dura tion of 60 min),once a day,3 days altogether. Immunohistochemical method was use d to display P53 protein expression in the cerebral cortex of elder rats with ce rebral ischemia-reperfusion injury. Results: Compared with sham-ope r ation group,the number of P53 immuno-reaction positive cells markedly increased in ischemia-reperfusion group ( P <0.05). In EA group, the number of P53 imm uno-reaction positive cells was significantly fewer than that of model group ( P <0.0 5),suggesting that EA could down-regulate P53 protein expression in rats with c erebral ischemia-reperfusion. Conclusion:EA could down-regulate P53 protein expression in cerebral ischemia rats, that may contribute to the actio n of EA in reducing cerebral neuronal apoptosis.