| New mutation detection system of repackaged λ gt11 DNA containing LacZ gene |
| |
| 引用本文： | 刘勇,曹佳,吴涛,杨录军,孙华明,杨明杰,钱频. New mutation detection system of repackaged λ gt11 DNA containing LacZ gene[J]. 中国人民解放军军医大学学报, 2002, 17(3) |
| |
| 作者姓名： | 刘勇 曹佳 吴涛 杨录军 孙华明 杨明杰 钱频 |
| |
| 作者单位： | Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China，Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China，Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China，Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China，Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China，Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China，Department of Hygiene Toxicology，Third Military Medical University，Chongqing 400038，China |
| |
| 基金项目： | National Science Foundation of China (NS-FC:No: 3880680，No: 39670643) |
| |
| 摘 要： | Objective: To establish a reformative detection system which has sound ability of providing information on molecular mutagenesis spectrum and the specificity of detection system of repackaged λ phage. Methods: LacZ gene, as mutational target gene and reporter gene, was applied into the detection system. The λ gt11 DNA treated with ENU (1-ethyl-1-nitrosourea) and 9-AA (9-aminoacridine) was repackaged in vitro. The packaged λ phage was then grown in E. coli Y1090 on a selective plate containing X-gel and IPTG. The survival and mutation frequencies were determined by counting the clear-plaque and blue-plaque, and the molecular mutation mechanism was studied by extracting and sequencing the LacZ gene of mutants. Results: The survival of repackaged λ phages treated with 9-AA and ENU apparently decreased in consistent dose-dependence. The mutation frequency of clear-plaque mutants showed a linear dose-related increase. The predominant mutations induced by 9-AA were ± 1 frameshift mutation, and 9-AA induc
|
| 关 键 词： | mutation λgt11 DNA LacZ gene 9-aminoacridine 1-ethyl-1-nitrosourea |
New mutation detection system of repackaged λ gt11 DNA containing LacZ gene |
| |
| LIU Yong,CAO Jia,WU Tao,YANG Lu-jun,SUN Hua-ming,YANG Ming-jie,QIAN Ping. New mutation detection system of repackaged λ gt11 DNA containing LacZ gene[J]. Journal of Medical Colleges of PLA(China), 2002, 17(3) |
| |
| Authors: | LIU Yong CAO Jia WU Tao YANG Lu-jun SUN Hua-ming YANG Ming-jie QIAN Ping |
| |
| Abstract: | |
| |
| Keywords: | mutation LacZ gene 9-aminoacridine 1-ethyl-1-nitrosourea |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
