Effects of low-level lead exposure on hypothalamic hormones and serum progesterone levels in pregnant guinea pigs.

Pregnant guinea pigs were given a daily oral dose of 0, 5.5, or 11 mg lead (as lead acetate) per kg body weight during days 22-52 or 22-62 of gestation. Maternal serum progesterone levels were measured at the end of treatment, as well as hypothalamic levels of gonadotropin-releasing hormone (GnRH) and somatostatin (SRIF) in both the mothers and fetuses. Lead-treated dams had lower serum concentrations of progesterone at the end of treatment than did vehicle-treated animals. This effect was statistically significant for the higher Pb dose only. Hypothalamic levels of GnRH and SRIF were reduced in a dose-dependent manner by lead treatment in both dams and fetuses. The reduction of SRIF levels in 52-day-old fetuses was particularly severe (92%) in the 11 mg group. However, neither litter size nor body and organ weights, including placental weight, of the dams and fetuses was significantly affected. The relevance of these hormonal decreases is unknown, but could include decreased reproductive capacity in both the dams and fetuses that does not become apparent until later in the life-cycle.