| 转染癌胚抗原基因的人树突状细胞疫苗表达的研究 |
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| 引用本文： | 马俊芬,黄幼田,杨洪艳,董子明. 转染癌胚抗原基因的人树突状细胞疫苗表达的研究[J]. 肿瘤基础与临床, 2003, 16(3): 164-165 |
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| 作者姓名： | 马俊芬 黄幼田 杨洪艳 董子明 |
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| 作者单位： | 郑州大学基础医学院病理生理学教研室,河南,郑州,450052 |
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| 摘 要： | 目的 探索使用质粒转导制备转基因DCs疫苗的方法。方法 采用细胞因子rhIL 4和rhGM CSF诱导培养法制备人外周血树突状细胞 ,用Lipofectine向人DCs转染含人CEA真核表达质粒。结果 转染组DCs培养上清CEA含量明显高于未转染组培养上清CEA含量。结论 人CEA真核表达质粒转染DCs可内源性表达CEA
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| 关 键 词： | 树突状细胞 癌胚抗原 基因转染 脂质体 |
| 文章编号： | 1003-1464(2003)03-0164-02 |
| 修稿时间： | 2002-12-13 |
The Expression of Dendritic Cell Transfected with Plasmid Carcinoembryonic Antigen |
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| MA Jun-fen,HUANG You-tian,YANG Hong-yan,et al. The Expression of Dendritic Cell Transfected with Plasmid Carcinoembryonic Antigen[J]. journal of basic and clinical oncology, 2003, 16(3): 164-165 |
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| Authors: | MA Jun-fen HUANG You-tian YANG Hong-yan et al |
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| Abstract: | Aim To prepare the vaccine of DCs transfected with plasimid carcinoembryonic antigen.Methods DC were generated from human peripheral blood.The plasmid encoding human carcinoembryonic antigen was transfected with Lipofectamine into human DCs.Results CEA expression level in the DC (pcDNA3-CEA +) culture supernatant is higher than in the DCs culture supernatant.Conclusions DCs transfected with human DC DNA3 CEA can endogenously express CEA. |
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| Keywords: | dendritic cell carcinoebryonic antigen gene transfection lipofectamine |
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