实时荧光定量PCR方法在沙门菌鉴定及筛选中的应用

目的在沙门菌鉴定中运用taqman技术建立荧光定量聚合酶链反应(PCR)的方法,并考评该方法的特异性、灵敏度、抗干扰性及重复性。方法根据沙门菌GenBank Accession NO DQ644631基因序列,设计并合成引物和荧光标记探针。建立实时荧光定量PCR反应体系,用沙门菌各种型别对该方法进行考评。结果实时荧光定量PCR技术在沙门菌鉴定中有良好的特异性、极高的灵敏度、较强的抗干扰性及重复性。结论在沙门菌的鉴定和筛选中实时荧光定量PCR方法准确、快速、简便,有很好的应用前景和研究价值。

Application of Real-time Fluorescence Quantitative PCR in Identification and Selection of Salmonella

Objective]To establish real-time fluorescence quantitative PCR in detection of samonella by using taqman technology,to evaluate the specificity,sensitivity,anti-interference and repeatability of this method.Methods]According to gene order of salmonella GenBank Accession NO DQ644631,primers and fluorescent-labeled probe were designed and synthesized.Real-time fluorescence quantitative PCR reaction system was established and evaluated by salmonella of various types.Results]Real-time fluorescence quantitati...