马鞭草醇提液小剂量时能显著增加紫杉醇的抗肿瘤活性

目的:研究马鞭草醇提液(EVO)联合紫杉醇(PTX)在体外和体内的抗肿瘤效果。方法:采用MTT法测定EVO与PTX单用以及联用对小鼠S180细胞的增殖抑制率;建立S180皮下种植瘤小鼠模型,观察EVO与PTX单用以及联用对小鼠皮下瘤的生长抑制作用。结果:体外实验发现在PTX的各个浓度下(3.75,7.50,15.00,30.00,60.00ng/ml),EVO20mg/ml联合后的细胞抑制效果均明显强于PTX单药。体内实验证实当EVO1g/kg与PTX10mg/kg联用时则对肿瘤生长产生了明显的抑制作用,与PTX10mg/kg单用相比具有显著性差异(P<0.05)。结论:体外和体内实验证实EVO在小剂量时能够显著增加PTX的抗肿瘤活性,这为提高临床常用化疗药物的疗效且减少增加剂量带来的不良反应提供了新的思路。

Synergistic anti-tumor effect of alcohol extract of verbena officinalis and paclitaxel

Objective:To explore the synergistic anti-tumor effect of alcohol extract of verbena officinalis(EVO) and paclitaxel (PTX). Methods:S180 cells were cultured in vitro, and the growth inhibition effects of the two drugs used respectively and in combination were detected by MTT methods. S180 transplant model was established in male ICR mice, and the tumor growth inhibition effects of the two drugs were calculated by measuring tumor volume in every two days. Results:In vitro experiment showed that cell growth was significantly inhibited by PTX when EVO was added concomitantly at a dose of 20 mg/ml. Moreover,in vivo experiment demonstrated that EVO(1 g/kg) efficiently enhanced the anti-tumor effect of PTX at a dose of 10 mg/kg. Conclusion:A subclinical dose of EVO could greatly improve the anti-cancer effect of paclitaxel,which provide a new strategy to amplify the efficiency of chemotherapeutics without increasing its dose.